Browsing FAS Scholarly Articles by Keyword "Cell Differentiation"
Now showing items 1-9 of 9
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Analysis of the Effects of Five Factors Relevant to In Vitro Chondrogenesis of Human Mesenchymal Stem Cells Using Factorial Design and High Throughput mRNA-Profiling
(Public Library of Science, 2014)The in vitro process of chondrogenic differentiation of mesenchymal stem cells for tissue engineering has been shown to require three-dimensional culture along with the addition of differentiation factors to the culture ... -
Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols
(Public Library of Science, 2014)Several protocols have been developed for human induced pluripotent stem cell neuronal differentiation. We compare several methods for forebrain cortical neuronal differentiation by assessing cell morphology, immunostaining ... -
Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells
(Public Library of Science, 2016)Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based ... -
The Evolution of Cell-to-Cell Communication in a Sporulating Bacterium
(Public Library of Science, 2012)Traditionally microorganisms were considered to be autonomous organisms that could be studied in isolation. However, over the last decades cell-to-cell communication has been found to be ubiquitous. By secreting molecular ... -
Germ Cells Are Not Required to Establish the Female Pathway in Mouse Fetal Gonads
(Public Library of Science, 2012)The fetal gonad is composed of a mixture of somatic cell lineages and germ cells. The fate of the gonad, male or female, is determined by a population of somatic cells that differentiate into Sertoli or granulosa cells and ... -
MARIS: Method for Analyzing RNA following Intracellular Sorting
(Public Library of Science, 2014)Transcriptional profiling is a key technique in the study of cell biology that is limited by the availability of reagents to uniquely identify specific cell types and isolate high quality RNA from them. We report a Method ... -
Phenotypic Heterogeneity and the Evolution of Bacterial Life Cycles
(Public Library of Science, 2016)Most bacteria live in colonies, where they often express different cell types. The ecological significance of these cell types and their evolutionary origin are often unknown. Here, we study the evolution of cell differentiation ... -
Polycomb-Mediated Repression and Sonic Hedgehog Signaling Interact to Regulate Merkel Cell Specification during Skin Development
(Public Library of Science, 2016)An increasing amount of evidence indicates that developmental programs are tightly regulated by the complex interplay between signaling pathways, as well as transcriptional and epigenetic processes. Here, we have uncovered ... -
Quantitative-Proteomic Comparison of Alpha and Beta Cells to Uncover Novel Targets for Lineage Reprogramming
(Public Library of Science, 2014)Type-1 diabetes (T1D) is an autoimmune disease in which insulin-secreting pancreatic beta cells are destroyed by the immune system. An emerging strategy to regenerate beta-cell mass is through transdifferentiation of ...