Browsing FAS Scholarly Articles by Keyword "Human"

Now showing items 1-10 of 10

    • The anticancer natural product ophiobolin A induces cytotoxicity by covalent modification of phosphatidylethanolamine 

      Chidley, Christopher; Trauger, Sunia A; Birsoy, Kıvanç; O'Shea, Erin K (eLife Sciences Publications, Ltd, 2016)
      Phenotypic screens allow the identification of small molecules with promising anticancer activity, but the difficulty in characterizing the mechanism of action of these compounds in human cells often undermines their value ...

    • Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4 

      Nicoludis, John M; Vogt, Bennett E; Green, Anna G; Schärfe, Charlotta PI; Marks, Debora S; Gaudet, Rachelle (eLife Sciences Publications, Ltd, 2016)
      Protocadherins (Pcdhs) are cell adhesion and signaling proteins used by neurons to develop and maintain neuronal networks, relying on trans homophilic interactions between their extracellular cadherin (EC) repeat domains. ...

    • Atypical calcium regulation of the PKD2-L1 polycystin ion channel 

      DeCaen, Paul G; Liu, Xiaowen; Abiria, Sunday; Clapham, David E (eLife Sciences Publications, Ltd, 2016)
      Native PKD2-L1 channel subunits are present in primary cilia and other restricted cellular spaces. Here we investigate the mechanism for the channel's unusual regulation by external calcium, and rationalize this behavior ...

    • Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies 

      Tang, Jonathan CY; Drokhlyansky, Eugene; Etemad, Behzad; Rudolph, Stephanie; Guo, Binggege; Wang, Sui; Ellis, Emily G; Li, Jonathan Z; Cepko, Constance L (eLife Sciences Publications, Ltd, 2016)
      The ability to detect and/or manipulate specific cell populations based upon the presence of intracellular protein epitopes would enable many types of studies and applications. Protein binders such as nanobodies (Nbs) can ...

    • Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma 

      Hettmer, Simone; Schinzel, Anna C; Tchessalova, Daria; Schneider, Michaela; Parker, Christina L; Bronson, Roderick T; Richards, Nigel GJ; Hahn, William C; Wagers, Amy J (eLife Sciences Publications, Ltd, 2015)
      Current therapies for sarcomas are often inadequate. This study sought to identify actionable gene targets by selective targeting of the molecular networks that support sarcoma cell proliferation. Silencing of asparagine ...

    • A general strategy to construct small molecule biosensors in eukaryotes 

      Feng, Justin; Jester, Benjamin W; Tinberg, Christine E; Mandell, Daniel J; Antunes, Mauricio S; Chari, Raj; Morey, Kevin J; Rios, Xavier; Medford, June I; Church, George M; Fields, Stanley; Baker, David (eLife Sciences Publications, Ltd, 2015)
      Biosensors for small molecules can be used in applications that range from metabolic engineering to orthogonal control of transcription. Here, we produce biosensors based on a ligand-binding domain (LBD) by using a method ...

    • MCU encodes the pore conducting mitochondrial calcium currents 

      Chaudhuri, Dipayan; Sancak, Yasemin; Mootha, Vamsi K; Clapham, David E (eLife Sciences Publications, Ltd, 2013)
      Mitochondrial calcium (Ca2+) import is a well-described phenomenon regulating cell survival and ATP production. Of multiple pathways allowing such entry, the mitochondrial Ca2+ uniporter is a highly Ca2+-selective channel ...

    • Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma 

      Tenente, Inês M; Hayes, Madeline N; Ignatius, Myron S; McCarthy, Karin; Yohe, Marielle; Sindiri, Sivasish; Gryder, Berkley; Oliveira, Mariana L; Ramakrishnan, Ashwin; Tang, Qin; Chen, Eleanor Y; Petur Nielsen, G; Khan, Javed; Langenau, David M (eLife Sciences Publications, Ltd, 2017)
      Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely ...

    • Sequencing the transcriptome of milk production: milk trumps mammary tissue 

      Lemay, Danielle G; Hovey, Russell C; Hartono, Stella R; Hinde, Katie; Smilowitz, Jennifer T; Ventimiglia, Frank; Schmidt, Kimberli A; Lee, Joyce WS; Islas-Trejo, Alma; Silva, Pedro Ivo; Korf, Ian; Medrano, Juan F; Barry, Peter A; German, J Bruce (BioMed Central, 2013)
      Background: Studies of normal human mammary gland development and function have mostly relied on cell culture, limited surgical specimens, and rodent models. Although RNA extracted from human milk has been used to assay ...

    • Sperm BerserKers 

      Clapham, David E (eLife Sciences Publications, Ltd, 2013)
      Human sperm cells rely on an unusual type of potassium ion channel.