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FRAP Analysis: Accounting for Bleaching during Image Capture 

Wu, Jun; Shekhar, Nandini; Lele, Pushkar Prakash; Lele, Tanmay P. (Public Library of Science, 2012)
The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the ...
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Positively Selected Sites in Cetacean Myoglobins Contribute to Protein Stability 

Dasmeh, Pouria; Serohijos, Adrian; Kepp, Kasper P.; Shakhnovich, Eugene Isaacovitch (Public Library of Science, 2013)
Since divergence ∼50 Ma ago from their terrestrial ancestors, cetaceans underwent a series of adaptations such as a ∼10–20 fold increase in myoglobin (Mb) concentration in skeletal muscle, critical for increasing oxygen ...
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Evolution after Introduction of a Novel Metabolic Pathway Consistently Leads to Restoration of Wild-Type Physiology 

Carroll, Sean; Marx, Christopher J (Public Library of Science, 2013)
Organisms cope with physiological stressors through acclimatizing mechanisms in the short-term and adaptive mechanisms over evolutionary timescales. During adaptation to an environmental or genetic perturbation, beneficial ...
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Genetic Characterization of smg-8 Mutants Reveals No Role in C. elegans Nonsense Mediated Decay 

Rosains, Jacqueline; Mango, Susan (Public Library of Science, 2012)
The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use ...
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The Methanol Dehydrogenase Gene, mxaF, as a Functional and Phylogenetic Marker for Proteobacterial Methanotrophs in Natural Environments 

Lau, Evan; Fisher, Meredith C.; Steudler, Paul A.; Cavanaugh, Colleen Marie (Public Library of Science, 2013)
The mxaF gene, coding for the large \((\alpha)\) subunit of methanol dehydrogenase, is highly conserved among distantly related methylotrophic species in the Alpha-, Beta- and Gammaproteobacteria. It is ubiquitous in ...
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Application of Palladium-Mediated 18F-Fluorination to PET Radiotracer Development: Overcoming Hurdles to Translation 

Kamlet, Adam; Neumann, Constanze Nicole; Lee, Eunsung; Carlin, Stephen M.; Moseley, Christian K.; Stephenson, Nickeisha A; Hooker, Jacob M; Ritter, Tobias (Public Library of Science, 2013)
New chemistry methods for the synthesis of radiolabeled small molecules have the potential to impact clinical positron emission tomography (PET) imaging, if they can be successfully translated. However, progression of ...
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Chromatin Modification by PSC Occurs at One PSC per Nucleosome and Does Not Require the Acidic Patch of Histone H2A 

Lo, Stanley M.; McElroy, Kyle Andrew; Francis, Nicole J. (Public Library of Science, 2012)
Chromatin architecture is regulated through both enzymatic and non-enzymatic activities. For example, the Polycomb Group (PcG) proteins maintain developmental gene silencing using an array of chromatin-based mechanisms. ...
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GW8510 Increases Insulin Expression in Pancreatic Alpha Cells through Activation of p53 Transcriptional Activity 

Fomina-Yadlin, Dina; Kubicek, Stefan; Vetere, Amedeo; He, Kaihui Hu; Schreiber, Stuart L.; Wagner, Bridget K. (Public Library of Science, 2012)
Background: Expression of insulin in terminally differentiated non-beta cell types in the pancreas could be important to treating type-1 diabetes. Previous findings led us to hypothesize involvement of kinase inhibition ...
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Metabolic Erosion Primarily Through Mutation Accumulation, and Not Tradeoffs, Drives Limited Evolution of Substrate Specificity in Escherichia coli 

Leiby, Nicholas; Marx, Christopher J. (Public Library of Science, 2014)
Evolutionary adaptation to a constant environment is often accompanied by specialization and a reduction of fitness in other environments. We assayed the ability of the Lenski Escherichia coli populations to grow on a range ...
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Predictive Value of Updating Framingham Risk Scores with Novel Risk Markers in the U.S. General Population 

Ferket, Bart S.; van Kempen, Bob J. H.; Hunink, M. G. Myriam; Agarwal, Isha; Kavousi, Maryam; Franco, Oscar H.; Steyerberg, Ewout W.; Max, Wendy; Fleischmann, Kirsten E. (Public Library of Science, 2014)
Background: According to population-based cohort studies CT coronary calcium score (CTCS), carotid intima-media thickness (cIMT), high-sensitivity C- reactive protein (CRP), and ankle-brachial index (ABI) are promising ...
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