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Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing
(Springer Science and Business Media LLC, 2021-12-09)
The targeted deletion, replacement, integration or inversion of genomic sequences could be used to study or treat human genetic diseases, but existing methods typically require double-strand DNA breaks (DSBs) that lead to ...
Geometric Deep Learning Enables 3D Kinematic Profiling Across Species and Environments
(Springer Science and Business Media LLC, 2021-04-19)
Comprehensive descriptions of animal behavior require precise measurements of 3D whole-body
movements. Although 2D approaches can track visible landmarks in restrictive environments,
performance drops significantly in ...
Bioinspired kirigami metasurfaces as assistive shoe grips
(Springer Science and Business Media LLC, 2020-06-01)
Falls and subsequent complications are major contributors to morbidity and mortality, especially in older adults. Here, by taking inspiration from claws and scales found in nature, we show that buckling kirigami structures ...
Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning
(Springer Science and Business Media LLC, 2021-06-28)
Task-Dependent Recruitment Across Ankle Extensor Muscles and Between Mechanical Demands Is Driven by the Metabolic Cost of Muscle Contraction
(The Royal Society, 2021-01-06)
The nervous system is faced with numerous strategies for recruiting a large number of motor units within and among muscle synergists to produce and control body movement. This is challenging, considering multiple combinations ...
Systemic Tumour Suppression via the Preferential Accumulation of Erythrocyte-Anchored Chemokine-Encapsulating Nanoparticles in Lung Metastases
(Springer Science and Business Media LLC, 2020-11-16)
Eliciting immune responses against primary tumours is hampered by their immunosuppressive microenvironment and by the greater inaccessibility of deeper intratumoural cells. Metastatic tumour cells are however exposed to ...
RNA Timestamps Identify the Age of Single Molecules in RNA Sequencing
(Springer Science and Business Media LLC, 2020-10-19)
Engineered pegRNAs improve prime editing efficiency
(Springer Science and Business Media LLC, 2021-10-04)
Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein ...
CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA
(Springer Science and Business Media LLC, 2022-04-04)
The all-protein cytosine base editor DdCBE uses TALE proteins and a double-stranded DNA-specific cytidine deaminase (DddA) to mediate targeted C•G-to-T•A editing. To improve editing efficiency and overcome the strict ...
High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells
(Springer Science and Business Media LLC, 2020-01-14)