Label-free single-cell protein quantification using a drop-based mix-and-read system

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Label-free single-cell protein quantification using a drop-based mix-and-read system

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Title: Label-free single-cell protein quantification using a drop-based mix-and-read system
Author: Abbaspourrad, Alireza; Zhang, Huidan; Tao, Ye; Cui, Naiwen; Asahara, Haruichi; Zhou, Ying; Yue, Dongxian; Koehler, Stephan A.; Ung, Lloyd W.; Heyman, John; Ren, Yukun; Ziblat, Roy; Chong, Shaorong; Weitz, David A.

Note: Order does not necessarily reflect citation order of authors.

Citation: Abbaspourrad, A., H. Zhang, Y. Tao, N. Cui, H. Asahara, Y. Zhou, D. Yue, et al. 2015. “Label-free single-cell protein quantification using a drop-based mix-and-read system.” Scientific Reports 5 (1): 12756. doi:10.1038/srep12756. http://dx.doi.org/10.1038/srep12756.
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Abstract: Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.
Published Version: doi:10.1038/srep12756
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522677/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:21461256
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