Label-free single-cell protein quantification using a drop-based mix-and-read system
DSpace/Manakin Repository
Label-free single-cell protein quantification using a drop-based mix-and-read system
| Title: | Label-free single-cell protein quantification using a drop-based mix-and-read system |
| Author: |
Abbaspourrad, Alireza; Zhang, Huidan; Tao, Ye; Cui, Naiwen; Asahara, Haruichi; Zhou, Ying; Yue, Dongxian; Koehler, Stephan A.; Ung, Lloyd W.; Heyman, John; Ren, Yukun; Ziblat, Roy; Chong, Shaorong; Weitz, David A.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Abbaspourrad, A., H. Zhang, Y. Tao, N. Cui, H. Asahara, Y. Zhou, D. Yue, et al. 2015. “Label-free single-cell protein quantification using a drop-based mix-and-read system.” Scientific Reports 5 (1): 12756. doi:10.1038/srep12756. http://dx.doi.org/10.1038/srep12756. |
| Full Text & Related Files: |
4522677.pdf (813.5Kb; PDF) 
|
| Abstract: | Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry. |
| Published Version: | doi:10.1038/srep12756 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522677/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:21461256 |
| Downloads of this work: |
This item appears in the following Collection(s)
-
FAS Scholarly Articles [15589]
Contact administrator regarding this item (to report mistakes or request changes)
