Label-free single-cell protein quantification using a drop-based mix-and-read system

 
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Tao, Ye
Cui, Naiwen
Asahara, Haruichi
Zhou, Ying
Yue, Dongxian
Ung, Lloyd W.
Ren, Yukun
Chong, Shaorong
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/srep12756
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Citation
Abbaspourrad, A., H. Zhang, Y. Tao, N. Cui, H. Asahara, Y. Zhou, D. Yue, et al. 2015. “Label-free single-cell protein quantification using a drop-based mix-and-read system.” Scientific Reports 5 (1): 12756. doi:10.1038/srep12756. http://dx.doi.org/10.1038/srep12756.
Abstract
Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522677/pdf/
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:21461256

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