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dc.contributor.authorBiggar, Kyle K.en_US
dc.contributor.authorWu, Cheng-Weien_US
dc.contributor.authorTessier, Shannon N.en_US
dc.contributor.authorZhang, Jingen_US
dc.contributor.authorPifferi, Fabienen_US
dc.contributor.authorPerret, Martineen_US
dc.contributor.authorStorey, Kenneth B.en_US
dc.date.accessioned2015-09-01T13:27:55Z
dc.date.issued2015en_US
dc.identifier.citationBiggar, Kyle K., Cheng-Wei Wu, Shannon N. Tessier, Jing Zhang, Fabien Pifferi, Martine Perret, and Kenneth B. Storey. 2015. “Modulation of Gene Expression in Key Survival Pathways During Daily Torpor in the Gray Mouse Lemur, Microcebus murinus.” Genomics, Proteomics & Bioinformatics 13 (2): 111-118. doi:10.1016/j.gpb.2015.03.001. http://dx.doi.org/10.1016/j.gpb.2015.03.001.en
dc.identifier.issn1672-0229en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:21461950
dc.description.abstractA variety of mammals employ torpor as an energy-saving strategy in environments of marginal or severe stress either on a daily basis during their inactive period or on a seasonal basis during prolonged multi-day hibernation. Recently, a few Madagascar lemur species have been identified as the only primates that exhibit torpor; one of these is the gray mouse lemur (Microcebus murinus). To explore the regulatory mechanisms that underlie daily torpor in a primate, we analyzed the expression of 28 selected genes that represent crucial survival pathways known to be involved in squirrel and bat hibernation. Array-based real-time PCR was used to compare gene expression in control (aroused) versus torpid lemurs in five tissues including the liver, kidney, skeletal muscle, heart, and brown adipose tissue. Significant differences in gene expression during torpor were revealed among genes involved in glycolysis, fatty acid metabolism, antioxidant defense, apoptosis, hypoxia signaling, and protein protection. The results showed upregulation of select genes primarily in liver and brown adipose tissue. For instance, both tissues showed elevated gene expression of peroxisome proliferator activated receptor gamma (ppargc), ferritin (fth1), and protein chaperones during torpor. Overall, the data show that the expression of only a few genes changed during lemur daily torpor, as compared with the broader expression changes reported for hibernation in ground squirrels. These results provide an indication that the alterations in gene expression required for torpor in lemurs are not as extensive as those needed for winter hibernation in squirrel models. However, identification of crucial genes with altered expression that support lemur torpor provides key targets to be explored and manipulated toward a goal of translational applications of inducible torpor as a treatment option in human biomedicine.en
dc.language.isoen_USen
dc.publisherElsevieren
dc.relation.isversionofdoi:10.1016/j.gpb.2015.03.001en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511780/pdf/en
dash.licenseLAAen_US
dc.subjectDaily torporen
dc.subjectPrimate hypometabolismen
dc.subjectPPAR gamma coactivatoren
dc.subjectFerritinen
dc.subjectChaperone proteinsen
dc.titleModulation of Gene Expression in Key Survival Pathways During Daily Torpor in the Gray Mouse Lemur, Microcebus murinusen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalGenomics, Proteomics & Bioinformaticsen
dash.depositing.authorTessier, Shannon N.en_US
dc.date.available2015-09-01T13:27:55Z
dc.identifier.doi10.1016/j.gpb.2015.03.001*
dash.contributor.affiliatedTessier, Shannon


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