A comparison of non-integrating reprogramming methods

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A comparison of non-integrating reprogramming methods

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Title: A comparison of non-integrating reprogramming methods
Author: Schlaeger, Thorsten M.; Daheron, Laurence Marie; Brickler, Thomas R; Entwisle, Samuel; Chan, Karrie; Cianci, Amelia; DeVine, Alexander; Ettenger, Andrew; Fitzgerald, Kelly; Godfrey, Michelle; Gupta, Dipti; McPherson, Jade; Malwadkar, Prerana; Gupta, Manav; Bell, Blair; Doi, Akiko; Jung, Namyoung; Li, Xin; Lynes, Maureen M.; Brookes, Emily; Cherry, Anne B C; Demirbas, Didem; Tsankov, Alexander; Zon, Leonard Ira; Rubin, Lee; Feinberg, Andrew P; Meissner, Alexander; Cowan, Chad A.; Daley, George Quentin

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Citation: Schlaeger, Thorsten M, Laurence Daheron, Thomas R Brickler, Samuel Entwisle, Karrie Chan, Amelia Cianci, Alexander DeVine, et al. 2014. “A Comparison of Non-Integrating Reprogramming Methods.” Nature Biotechnology 33 (1) (December 1): 58–63. doi:10.1038/nbt.3070.
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Abstract: Human induced pluripotent stem cells (hiPSCs1, 2, 3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)4, episomal (Epi)5 and mRNA transfection mRNA6 methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.
Published Version: doi:10.1038/nbt.3070
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:22556353
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