A comparison of non-integrating reprogramming methods
Brickler, Thomas R
Cherry, Anne B C
Feinberg, Andrew P
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CitationSchlaeger, Thorsten M, Laurence Daheron, Thomas R Brickler, Samuel Entwisle, Karrie Chan, Amelia Cianci, Alexander DeVine, et al. 2014. “A Comparison of Non-Integrating Reprogramming Methods.” Nature Biotechnology 33 (1) (December 1): 58–63. doi:10.1038/nbt.3070.
AbstractHuman induced pluripotent stem cells (hiPSCs1, 2, 3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)4, episomal (Epi)5 and mRNA transfection mRNA6 methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:22556353
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