Acute myocardial infarction activates distinct inflammation and proliferation pathways in circulating monocytes, prior to recruitment, and identified through conserved transcriptional responses in mice and humans
View/ Open
Author
Ruparelia, Neil
Lee, Regent
Chai, Joshua T.
Dall'Armellina, Erica
McAndrew, Debra
Digby, Janet E.
Forfar, J. Colin
Prendergast, Bernard D.
Kharbanda, Rajesh K.
Banning, Adrian P.
Neubauer, Stefan
Lygate, Craig A.
Channon, Keith M.
Haining, Nicholas W.
Choudhury, Robin P.
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1093/eurheartj/ehv195Metadata
Show full item recordCitation
Ruparelia, N., J. Godec, R. Lee, J. T. Chai, E. Dall'Armellina, D. McAndrew, J. E. Digby, et al. 2015. “Acute myocardial infarction activates distinct inflammation and proliferation pathways in circulating monocytes, prior to recruitment, and identified through conserved transcriptional responses in mice and humans.” European Heart Journal 36 (29): 1923-1934. doi:10.1093/eurheartj/ehv195. http://dx.doi.org/10.1093/eurheartj/ehv195.Abstract
Aims Monocytes play critical roles in tissue injury and repair following acute myocardial infarction (AMI). Specifically targeting inflammatory monocytes in experimental models leads to reduced infarct size and improved healing. However, data from humans are sparse, and it remains unclear whether monocytes play an equally important role in humans. The aim of this study was to investigate whether the monocyte response following AMI is conserved between humans and mice and interrogate patterns of gene expression to identify regulated functions. Methods and results Thirty patients (AMI) and 24 control patients (stable coronary atherosclerosis) were enrolled. Female C57BL/6J mice (n = 6/group) underwent AMI by surgical coronary ligation. Myocardial injury was quantified by magnetic resonance imaging (human) and echocardiography (mice). Peripheral monocytes were isolated at presentation and at 48 h. RNA from separated monocytes was hybridized to Illumina beadchips. Acute myocardial infarction resulted in a significant peripheral monocytosis in both species that positively correlated with the extent of myocardial injury. Analysis of the monocyte transcriptome following AMI demonstrated significant conservation and identified inflammation and mitosis as central processes to this response. These findings were validated in both species. Conclusions: Our findings show that the monocyte transcriptome is conserved between mice and humans following AMI. Patterns of gene expression associated with inflammation and proliferation appear to be switched on prior to their infiltration of injured myocardium suggesting that the specific targeting of inflammatory and proliferative processes in these immune cells in humans are possible therapeutic strategies. Importantly, they could be effective in the hours after AMI.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571177/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:22856836
Collections
- FAS Scholarly Articles [18256]
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)