Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction

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Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction

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Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
Author: Vicente-Steijn, Rebecca; Scherptong, Roderick W. C.; Kruithof, Boudewijn P. T.; Duim, Sjoerd N.; Goumans, Marie Jose T. H.; Wisse, Lambertus J.; Zhou, Bin; Pu, William T.; Poelmann, Robert E.; Schalij, Martin J.; Tallquist, Michelle D.; Gittenberger-de Groot, Adriana C.; Jongbloed, Monique RM

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Citation: Vicente-Steijn, R., R. W. C. Scherptong, B. P. T. Kruithof, S. N. Duim, M. J. T. H. Goumans, L. J. Wisse, B. Zhou, et al. 2015. “Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction.” PLoS ONE 10 (9): e0136025. doi:10.1371/journal.pone.0136025. http://dx.doi.org/10.1371/journal.pone.0136025.
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Abstract: Background: Morphological and functional differences of the right and left ventricle are apparent in the adult human heart. A differential contribution of cardiac fibroblasts and smooth muscle cells (populations of epicardium-derived cells) to each ventricle may account for part of the morphological-functional disparity. Here we studied the relation between epicardial derivatives and the development of compact ventricular myocardium. Results: Wildtype and Wt1CreERT2/+ reporter mice were used to study WT-1 expressing cells, and Tcf21lacZ/+ reporter mice and PDGFRα-/-;Tcf21LacZ/+ mice to study the formation of the cardiac fibroblast population. After covering the heart, intramyocardial WT-1+ cells were first observed at the inner curvature, the right ventricular postero-lateral wall and left ventricular apical wall. Later, WT-1+ cells were present in the walls of both ventricles, but significantly more pronounced in the left ventricle. Tcf21-LacZ + cells followed the same distribution pattern as WT-1+ cells but at later stages, indicating a timing difference between these cell populations. Within the right ventricle, WT-1+ and Tcf21-lacZ+ cell distribution was more pronounced in the posterior inlet part. A gradual increase in myocardial wall thickness was observed early in the left ventricle and at later stages in the right ventricle. PDGFRα-/-;Tcf21LacZ/+ mice showed deficient epicardium, diminished number of Tcf21-LacZ + cells and reduced ventricular compaction. Conclusions: During normal heart development, spatio-temporal differences in contribution of WT-1 and Tcf21-LacZ + cells to right versus left ventricular myocardium occur parallel to myocardial thickening. These findings may relate to lateralized differences in ventricular (patho)morphology in humans.
Published Version: doi:10.1371/journal.pone.0136025
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577115/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:22856983
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