Thiostrepton interacts covalently with Rpt subunits of the 19S proteasome and proteasome substrates

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Thiostrepton interacts covalently with Rpt subunits of the 19S proteasome and proteasome substrates

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Title: Thiostrepton interacts covalently with Rpt subunits of the 19S proteasome and proteasome substrates
Author: Sandu, Cristinel; Chandramouli, Nagaranjan; Glickman, Joseph Fraser; Molina, Henrik; Kuo, Chueh-Ling; Kukushkin, Nikolay; Goldberg, Alfred L; Steller, Hermann

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Citation: Sandu, Cristinel, Nagaranjan Chandramouli, Joseph Fraser Glickman, Henrik Molina, Chueh-Ling Kuo, Nikolay Kukushkin, Alfred L Goldberg, and Hermann Steller. 2015. “Thiostrepton interacts covalently with Rpt subunits of the 19S proteasome and proteasome substrates.” Journal of Cellular and Molecular Medicine 19 (9): 2181-2192. doi:10.1111/jcmm.12602. http://dx.doi.org/10.1111/jcmm.12602.
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Abstract: Here, we report a novel mechanism of proteasome inhibition mediated by Thiostrepton (Thsp), which interacts covalently with Rpt subunits of the 19S proteasome and proteasome substrates. We identified Thsp in a cell-based high-throughput screen using a fluorescent reporter sensitive to degradation by the ubiquitin–proteasome pathway. Thiostrepton behaves as a proteasome inhibitor in several paradigms, including cell-based reporters, detection of global ubiquitination status, and proteasome-mediated labile protein degradation. In vitro, Thsp does not block the chymotrypsin activity of the 26S proteasome. In a cell-based IκBα degradation assay, Thsp is a slow inhibitor and 4 hrs of treatment achieves the same effects as MG-132 at 30 min. We show that Thsp forms covalent adducts with proteins in human cells and demonstrate their nature by mass spectrometry. Furthermore, the ability of Thsp to interact covalently with the cysteine residues is essential for its proteasome inhibitory function. We further show that a Thsp modified peptide cannot be degraded by proteasomes in vitro. Importantly, we demonstrate that Thsp binds covalently to Rpt subunits of the 19S regulatory particle and forms bridges with a proteasome substrate. Taken together, our results uncover an important role of Thsp in 19S proteasome inhibition.
Published Version: doi:10.1111/jcmm.12602
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568923/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:22856985
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