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dc.contributor.authorCohen, Keira A.en_US
dc.contributor.authorAbeel, Thomasen_US
dc.contributor.authorManson McGuire, Abigailen_US
dc.contributor.authorDesjardins, Christopher A.en_US
dc.contributor.authorMunsamy, Vanishaen_US
dc.contributor.authorShea, Terrance P.en_US
dc.contributor.authorWalker, Bruce J.en_US
dc.contributor.authorBantubani, Nonkqubelaen_US
dc.contributor.authorAlmeida, Deepak V.en_US
dc.contributor.authorAlvarado, Luciaen_US
dc.contributor.authorChapman, Sinéad B.en_US
dc.contributor.authorMvelase, Nomonde R.en_US
dc.contributor.authorDuffy, Eamon Y.en_US
dc.contributor.authorFitzgerald, Michael G.en_US
dc.contributor.authorGovender, Pamlaen_US
dc.contributor.authorGujja, Sharvarien_US
dc.contributor.authorHamilton, Susannaen_US
dc.contributor.authorHowarth, Clintonen_US
dc.contributor.authorLarimer, Jeffrey D.en_US
dc.contributor.authorMaharaj, Kashmeelen_US
dc.contributor.authorPearson, Matthew D.en_US
dc.contributor.authorPriest, Margaret E.en_US
dc.contributor.authorZeng, Qiandongen_US
dc.contributor.authorPadayatchi, Nesrien_US
dc.contributor.authorGrosset, Jacquesen_US
dc.contributor.authorYoung, Sarah K.en_US
dc.contributor.authorWortman, Jenniferen_US
dc.contributor.authorMlisana, Koleka P.en_US
dc.contributor.authorO'Donnell, Max R.en_US
dc.contributor.authorBirren, Bruce W.en_US
dc.contributor.authorBishai, William R.en_US
dc.contributor.authorPym, Alexander S.en_US
dc.contributor.authorEarl, Ashlee M.en_US
dc.date.accessioned2015-11-03T15:57:43Z
dc.date.issued2015en_US
dc.identifier.citationCohen, K. A., T. Abeel, A. Manson McGuire, C. A. Desjardins, V. Munsamy, T. P. Shea, B. J. Walker, et al. 2015. “Evolution of Extensively Drug-Resistant Tuberculosis over Four Decades: Whole Genome Sequencing and Dating Analysis of Mycobacterium tuberculosis Isolates from KwaZulu-Natal.” PLoS Medicine 12 (9): e1001880. doi:10.1371/journal.pmed.1001880. http://dx.doi.org/10.1371/journal.pmed.1001880.en
dc.identifier.issn1549-1277en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23473914
dc.description.abstractBackground: The continued advance of antibiotic resistance threatens the treatment and control of many infectious diseases. This is exemplified by the largest global outbreak of extensively drug-resistant (XDR) tuberculosis (TB) identified in Tugela Ferry, KwaZulu-Natal, South Africa, in 2005 that continues today. It is unclear whether the emergence of XDR-TB in KwaZulu-Natal was due to recent inadequacies in TB control in conjunction with HIV or other factors. Understanding the origins of drug resistance in this fatal outbreak of XDR will inform the control and prevention of drug-resistant TB in other settings. In this study, we used whole genome sequencing and dating analysis to determine if XDR-TB had emerged recently or had ancient antecedents. Methods and Findings: We performed whole genome sequencing and drug susceptibility testing on 337 clinical isolates of Mycobacterium tuberculosis collected in KwaZulu-Natal from 2008 to 2013, in addition to three historical isolates, collected from patients in the same province and including an isolate from the 2005 Tugela Ferry XDR outbreak, a multidrug-resistant (MDR) isolate from 1994, and a pansusceptible isolate from 1995. We utilized an array of whole genome comparative techniques to assess the relatedness among strains, to establish the order of acquisition of drug resistance mutations, including the timing of acquisitions leading to XDR-TB in the LAM4 spoligotype, and to calculate the number of independent evolutionary emergences of MDR and XDR. Our sequencing and analysis revealed a 50-member clone of XDR M. tuberculosis that was highly related to the Tugela Ferry XDR outbreak strain. We estimated that mutations conferring isoniazid and streptomycin resistance in this clone were acquired 50 y prior to the Tugela Ferry outbreak (katG S315T [isoniazid]; gidB 130 bp deletion [streptomycin]; 1957 [95% highest posterior density (HPD): 1937–1971]), with the subsequent emergence of MDR and XDR occurring 20 y (rpoB L452P [rifampicin]; pncA 1 bp insertion [pyrazinamide]; 1984 [95% HPD: 1974–1992]) and 10 y (rpoB D435G [rifampicin]; rrs 1400 [kanamycin]; gyrA A90V [ofloxacin]; 1995 [95% HPD: 1988–1999]) prior to the outbreak, respectively. We observed frequent de novo evolution of MDR and XDR, with 56 and nine independent evolutionary events, respectively. Isoniazid resistance evolved before rifampicin resistance 46 times, whereas rifampicin resistance evolved prior to isoniazid only twice. We identified additional putative compensatory mutations to rifampicin in this dataset. One major limitation of this study is that the conclusions with respect to ordering and timing of acquisition of mutations may not represent universal patterns of drug resistance emergence in other areas of the globe. Conclusions: In the first whole genome-based analysis of the emergence of drug resistance among clinical isolates of M. tuberculosis, we show that the ancestral precursor of the LAM4 XDR outbreak strain in Tugela Ferry gained mutations to first-line drugs at the beginning of the antibiotic era. Subsequent accumulation of stepwise resistance mutations, occurring over decades and prior to the explosion of HIV in this region, yielded MDR and XDR, permitting the emergence of compensatory mutations. Our results suggest that drug-resistant strains circulating today reflect not only vulnerabilities of current TB control efforts but also those that date back 50 y. In drug-resistant TB, isoniazid resistance was overwhelmingly the initial resistance mutation to be acquired, which would not be detected by current rapid molecular diagnostics employed in South Africa that assess only rifampicin resistance.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pmed.1001880en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587932/pdf/en
dash.licenseLAAen_US
dc.titleEvolution of Extensively Drug-Resistant Tuberculosis over Four Decades: Whole Genome Sequencing and Dating Analysis of Mycobacterium tuberculosis Isolates from KwaZulu-Natalen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Medicineen
dash.depositing.authorCohen, Keira A.en_US
dc.date.available2015-11-03T15:57:43Z
dc.identifier.doi10.1371/journal.pmed.1001880*
dash.authorsorderedfalse
dash.contributor.affiliatedCohen, Keira A.


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