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dc.contributor.authorLin, Jia-Renen_US
dc.contributor.authorFallahi-Sichani, Mohammaden_US
dc.contributor.authorSorger, Peter K.en_US
dc.date.accessioned2015-11-03T15:57:46Z
dc.date.issued2015en_US
dc.identifier.citationLin, Jia-Ren, Mohammad Fallahi-Sichani, and Peter K. Sorger. 2015. “Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.” Nature Communications 6 (1): 8390. doi:10.1038/ncomms9390. http://dx.doi.org/10.1038/ncomms9390.en
dc.identifier.issn2041-1723en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23473921
dc.description.abstractSingle-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signalling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-colour staining alternates with chemical inactivation of fluorophores to progressively build a multichannel image. Because CycIF uses standard reagents and instrumentation and is no more expensive than conventional IF, it is suitable for high-throughput assays and screening applications.en
dc.language.isoen_USen
dc.publisherNature Pub. Groupen
dc.relation.isversionofdoi:10.1038/ncomms9390en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587398/pdf/en
dash.licenseLAAen_US
dc.titleHighly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence methoden
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature Communicationsen
dash.depositing.authorLin, Jia-Renen_US
dc.date.available2015-11-03T15:57:46Z
dc.identifier.doi10.1038/ncomms9390*
dash.contributor.affiliatedLin, Jia-Ren
dash.contributor.affiliatedSorger, Peter


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