RGS4 inhibits angiotensin II signaling and macrophage localization during renal reperfusion injury independent of vasospasm
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Author
Pang, Paul
Jin, Xiaohua
Proctor, Brandon M.
Farley, Michelle
Roy, Nilay
Chin, Matthew S.
Vollmann, Elisabeth
Perro, Mario
Hoffman, Ryan J.
Chung, Joseph
Chauhan, Nikita
Mistri, Murti
Muslin, Anthony J.
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1038/ki.2014.364Metadata
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Pang, P., X. Jin, B. M. Proctor, M. Farley, N. Roy, M. S. Chin, U. H. von Andrian, et al. 2014. “RGS4 inhibits angiotensin II signaling and macrophage localization during renal reperfusion injury independent of vasospasm.” Kidney international 87 (4): 771-783. doi:10.1038/ki.2014.364. http://dx.doi.org/10.1038/ki.2014.364.Abstract
Vascular inflammation is a major contributor to the severity of acute kidney injury. In the context of vasospasm-independent reperfusion injury we studied the potential anti-inflammatory role of the Gα-related RGS protein, RGS4. Transgenic RGS4 mice were resistant to 25 minute injury, although post-ischemic renal arteriolar diameter was equal to the wild type early after injury. A 10 minute unilateral injury was performed to study reperfusion without vasospasm. Eighteen hours after injury blood flow was decreased in the inner cortex of wild type mice with preservation of tubular architecture. Angiotensin II levels in the kidneys of wild type and transgenic mice were elevated in a sub-vasoconstrictive range 12 and 18 hours after injury. Angiotensin II stimulated pre-glomerular vascular smooth muscle cells (VSMC) to secrete the macrophage chemoattractant, RANTES; a process decreased by angiotensin II R2 (AT2) inhibition. However, RANTES increased when RGS4 expression was suppressed implicating Gα protein activation in an AT2-RGS4-dependent pathway. RGS4 function, specific to VSMC, was tested in a conditional VSMC-specific RGS4 knockout showing high macrophage density by T2 MRI compared to transgenic and non-transgenic mice after the 10 minute injury. Arteriolar diameter of this knockout was unchanged at successive time points after injury. Thus, RGS4 expression, specific to renal VSMC, inhibits angiotensin II-mediated cytokine signaling and macrophage recruitment during reperfusion, distinct from vasomotor regulation.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382433/pdf/Terms of Use
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