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dc.contributor.authorDutta, Parthaen_US
dc.contributor.authorHoyer, Friedrich Felixen_US
dc.contributor.authorGrigoryeva, Lubov S.en_US
dc.contributor.authorSager, Hendrik B.en_US
dc.contributor.authorLeuschner, Florianen_US
dc.contributor.authorCourties, Gabrielen_US
dc.contributor.authorBorodovsky, Annaen_US
dc.contributor.authorNovobrantseva, Tatianaen_US
dc.contributor.authorRuda, Vera M.en_US
dc.contributor.authorFitzgerald, Kevinen_US
dc.contributor.authorIwamoto, Yoshikoen_US
dc.contributor.authorWojtkiewicz, Gregoryen_US
dc.contributor.authorSun, Yuanen_US
dc.contributor.authorDa Silva, Nicolasen_US
dc.contributor.authorLibby, Peteren_US
dc.contributor.authorAnderson, Daniel G.en_US
dc.contributor.authorSwirski, Filip K.en_US
dc.contributor.authorWeissleder, Ralphen_US
dc.contributor.authorNahrendorf, Matthiasen_US
dc.date.accessioned2015-11-03T15:58:00Z
dc.date.issued2015en_US
dc.identifier.citationDutta, P., F. F. Hoyer, L. S. Grigoryeva, H. B. Sager, F. Leuschner, G. Courties, A. Borodovsky, et al. 2015. “Macrophages retain hematopoietic stem cells in the spleen via VCAM-1.” The Journal of Experimental Medicine 212 (4): 497-512. doi:10.1084/jem.20141642. http://dx.doi.org/10.1084/jem.20141642.en
dc.identifier.issn0022-1007en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23473961
dc.description.abstractSplenic myelopoiesis provides a steady flow of leukocytes to inflamed tissues, and leukocytosis correlates with cardiovascular mortality. Yet regulation of hematopoietic stem cell (HSC) activity in the spleen is incompletely understood. Here, we show that red pulp vascular cell adhesion molecule 1 (VCAM-1)+ macrophages are essential to extramedullary myelopoiesis because these macrophages use the adhesion molecule VCAM-1 to retain HSCs in the spleen. Nanoparticle-enabled in vivo RNAi silencing of the receptor for macrophage colony stimulation factor (M-CSFR) blocked splenic macrophage maturation, reduced splenic VCAM-1 expression and compromised splenic HSC retention. Both, depleting macrophages in CD169 iDTR mice or silencing VCAM-1 in macrophages released HSCs from the spleen. When we silenced either VCAM-1 or M-CSFR in mice with myocardial infarction or in ApoE−/− mice with atherosclerosis, nanoparticle-enabled in vivo RNAi mitigated blood leukocytosis, limited inflammation in the ischemic heart, and reduced myeloid cell numbers in atherosclerotic plaques.en
dc.language.isoen_USen
dc.publisherThe Rockefeller University Pressen
dc.relation.isversionofdoi:10.1084/jem.20141642en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387283/pdf/en
dash.licenseLAAen_US
dc.titleMacrophages retain hematopoietic stem cells in the spleen via VCAM-1en
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalThe Journal of Experimental Medicineen
dash.depositing.authorDutta, Parthaen_US
dc.date.available2015-11-03T15:58:00Z
dc.identifier.doi10.1084/jem.20141642*
dash.authorsorderedfalse
dash.contributor.affiliatedDa Silva, Nicolas
dash.contributor.affiliatedDutta, Partha
dash.contributor.affiliatedHoyer, Friedrich
dash.contributor.affiliatedAnderson, Daniel
dash.contributor.affiliatedCourties, Gabriel
dash.contributor.affiliatedSwirski, Filip
dash.contributor.affiliatedNahrendorf, Matthias
dash.contributor.affiliatedLibby, Peter
dash.contributor.affiliatedWeissleder, Ralph


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