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dc.contributor.authorWen, Heen_US
dc.contributor.authorLee, Tacken_US
dc.contributor.authorYou, Sungyongen_US
dc.contributor.authorPark, Soo-Hwanen_US
dc.contributor.authorSong, Hosooken_US
dc.contributor.authorEilber, Karyn S.en_US
dc.contributor.authorAnger, Jennifer T.en_US
dc.contributor.authorFreeman, Michael R.en_US
dc.contributor.authorPark, Sunghyouken_US
dc.contributor.authorKim, Jayoungen_US
dc.date.accessioned2015-11-03T15:58:31Z
dc.date.issued2014en_US
dc.identifier.citationWen, He, Tack Lee, Sungyong You, Soo-Hwan Park, Hosook Song, Karyn S. Eilber, Jennifer T. Anger, Michael R. Freeman, Sunghyouk Park, and Jayoung Kim. 2014. “Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers.” Journal of Proteome Research 14 (1): 541-548. doi:10.1021/pr5007729. http://dx.doi.org/10.1021/pr5007729.en
dc.identifier.issn1535-3893en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23474047
dc.description.abstractInterstitial cystitis/painful bladder syndrome (IC) is a chronic syndrome of unknown etiology that presents with bladder pain, urinary frequency, and urgency. The lack of specific biomarkers and a poor understanding of underlying molecular mechanisms present challenges for disease diagnosis and therapy. The goals of this study were to identify noninvasive biomarker candidates for IC from urine specimens and to potentially gain new insight into disease mechanisms using a nuclear magnetic resonance (NMR)-based global metabolomics analysis of urine from female IC patients and controls. Principal component analysis (PCA) suggested that the urinary metabolome of IC and controls was clearly different, with 140 NMR peaks significantly altered in IC patients (FDR < 0.05) compared to that in controls. On the basis of strong correlation scores, fifteen metabolite peaks were nominated as the strongest signature of IC. Among those signals that were higher in the IC group, three peaks were annotated as tyramine, the pain-related neuromodulator. Two peaks were annotated as 2-oxoglutarate. Levels of tyramine and 2-oxoglutarate were significantly elevated in urine specimens of IC subjects. An independent analysis using mass spectrometry also showed significantly increased levels of tyramine and 2-oxoglutarate in IC patients compared to controls. Functional studies showed that 2-oxoglutarate, but not tyramine, retarded growth of normal bladder epithelial cells. These preliminary findings suggest that analysis of urine metabolites has promise in biomarker development in the context of IC.en
dc.language.isoen_USen
dc.publisherAmerican Chemical Societyen
dc.relation.isversionofdoi:10.1021/pr5007729en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286193/pdf/en
dash.licenseLAAen_US
dc.subjectArticleen
dc.subjectMetabolomicsen
dc.subjectNMRen
dc.subjectmetabolitesen
dc.subjectinterstitial cystitisen
dc.subjectbladderen
dc.subjectbiomarkeren
dc.titleUrinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkersen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalJournal of Proteome Researchen
dash.depositing.authorFreeman, Michael R.en_US
dc.date.available2015-11-03T15:58:31Z
dc.identifier.doi10.1021/pr5007729*
dash.authorsorderedfalse
dash.contributor.affiliatedFreeman, Michael R.
dash.contributor.affiliatedKim, Jayoung


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