Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides

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Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides

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Title: Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides
Author: Kamat, Neha P; Tobé, Sylvia; Hill, Ian T; Szostak, Jack W

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Citation: Kamat, Neha P, Sylvia Tobé, Ian T Hill, and Jack W Szostak. 2015. “Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides.” Angewandte Chemie (International Ed. in English) 54 (40): 11735-11739. doi:10.1002/anie.201505742. http://dx.doi.org/10.1002/anie.201505742.
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Abstract: Cooperative interactions between RNA and vesicle membranes on the prebiotic earth may have led to the emergence of primitive cells. The membrane surface offers a potential platform for the catalysis of reactions involving RNA, but this scenario relies upon the existence of a simple mechanism by which RNA could become associated with protocell membranes. Here, we show that electrostatic interactions provided by short, basic, amphipathic peptides can be harnessed to drive RNA binding to both zwitterionic phospholipid and anionic fatty acid membranes. We show that the association of cationic molecules with phospholipid vesicles can enhance the local positive charge on a membrane and attract RNA polynucleotides. This phenomenon can be reproduced with amphipathic peptides as short as three amino acids. Finally, we show that peptides can cross bilayer membranes to localize encapsulated RNA. This mechanism of polynucleotide confinement could have been important for primitive cellular evolution.
Published Version: doi:10.1002/anie.201505742
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600236/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:23474058
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