LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
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CitationAlbeanu, Dinu F., Edward Soucy, Tomokazu F. Sato, Markus Meister, Venkatesh N. Murthy. 2008. LED arrays as cost effective and efficient light sources for widefield microscopy. PLoS ONE 3(5).
AbstractNew developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the
life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for
fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we
describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes
(LEDs). We also provide examples of its applicability to biological fluorescence imaging.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:2350197
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