LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy

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LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy

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Title: LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy
Author: Murthy, Venkatesh; Sato, Tomokazu F.; Albeanu, Dinu F.; Soucy, Edward; Meister, Markus

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Citation: Albeanu, Dinu F., Edward Soucy, Tomokazu F. Sato, Markus Meister, Venkatesh N. Murthy. 2008. LED arrays as cost effective and efficient light sources for widefield microscopy. PLoS ONE 3(5).
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Abstract: New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the
life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for
fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we
describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes
(LEDs). We also provide examples of its applicability to biological fluorescence imaging.
Published Version: doi:10.1371/journal.pone.0002146
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:2350197
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