Show simple item record

dc.contributor.authorSherman, David Joseph
dc.contributor.authorLazarus, Michael B.
dc.contributor.authorMurphy, Lea
dc.contributor.authorLiu, Charles Xin
dc.contributor.authorKahne, Suzanne Walker
dc.contributor.authorRuiz, Natividad
dc.contributor.authorKahne, Daniel
dc.date.accessioned2015-11-20T18:54:07Z
dc.date.issued2014
dc.identifierQuick submit: 2015-11-05T13:35:30-05:00
dc.identifier.citationSherman, David J., Michael B. Lazarus, Lea Murphy, Charles Liu, Suzanne Walker, Natividad Ruiz, and Daniel Kahne. 2014. “Decoupling Catalytic Activity from Biological Function of the ATPase That Powers Lipopolysaccharide Transport.” Proc Natl Acad Sci USA 111 (13) (March 17): 4982–4987. doi:10.1073/pnas.1323516111.en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23597719
dc.description.abstractGram-negative bacteria contain an unusual outer membrane that prevents the entry of most currently available antibiotics. This membrane contains a complex glycolipid, LPS, on the exterior. It is not understood how such a large molecule, which can contain hundreds of sugars and six fatty acyl chains, is transported across the cell envelope from its site of synthesis in the cytoplasmic membrane to the cell surface. Using a combination of genetics, biochemistry, and structural biology, we characterized residues in the protein that powers LPS transport to gain mechanistic insight into how ATP hydrolysis is coupled to the biological function of the transporter. These tools help us understand how to design antibiotics targeting this essential pathway.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherProceedings of the National Academy of Sciencesen_US
dc.relation.isversionofdoi:10.1073/pnas.1323516111en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977253/en_US
dash.licenseOAP
dc.subjectABC transporteren_US
dc.subjectouter membraneen_US
dc.subjectGram-negative bacteriaen_US
dc.subjectmembrane biogenesisen_US
dc.subjectantibiotic targeten_US
dc.titleDecoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transporten_US
dc.typeJournal Articleen_US
dc.date.updated2015-11-05T18:35:31Z
dc.description.versionAccepted Manuscripten_US
dc.rights.holderSherman D, Lazarus MB, Murphy L, Liu C, Walker S, Ruiz N, Kahne D.
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dash.depositing.authorKahne, Daniel
dc.date.available2015-11-20T18:54:07Z
dc.identifier.doi10.1073/pnas.1323516111*
dash.authorsorderedfalse
dash.contributor.affiliatedLiu, Charles
dash.contributor.affiliatedKahne, Suzanne
dash.contributor.affiliatedSherman, David
dash.contributor.affiliatedKahne, Daniel


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record