An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis

DSpace/Manakin Repository

An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis

Citable link to this page

 

 
Title: An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis
Author: Reiberger, Thomas; Chen, Yunching; Ramjiwan, Rakesh R.; Hato, Tai; Fan, Christopher; Samuel, Rekha; Roberge, Sylvie; Huang, Peigen; Lauwers, Gregory Y.; Zhu, Andrew Xiuxuan; Bardeesy, Nabeel; Jain, Rakesh K.; Duda, Dan Gabriel

Note: Order does not necessarily reflect citation order of authors.

Citation: Reiberger, Thomas, Yunching Chen, Rakesh R Ramjiawan, Tai Hato, Christopher Fan, Rekha Samuel, Sylvie Roberge, et al. 2015. “An Orthotopic Mouse Model of Hepatocellular Carcinoma with Underlying Liver Cirrhosis.” Nature Protocols 10 (8) (July 23): 1264–1274. doi:10.1038/nprot.2015.080.
Full Text & Related Files:
Abstract: Subcutaneous xenografts have been used for decades to study hepatocellular carcinoma (HCC). These models do not reproduce the specific pathophysiological features of HCCs, which occur in cirrhotic livers showing pronounced necroinflammation, abnormal angiogenesis, and extensive fibrosis. As these features are critical for studying the role of the pathologic host microenvironment in tumor initiation, progression, and treatment response, alternative HCC models are desirable. Here, we describe a syngeneic orthotopic HCC model in immunocompetent mice with liver cirrhosis induced by carbon tetrachloride (CCl4) that recapitulates key features of human HCC. Induction of significant hepatic fibrosis requires 12 weeks of CCl4 administration. Intrahepatic implantation of murine HCC cell lines requires 30 minutes per mouse. Tumor growth varies by tumor cell line and mouse strain used. Alternatively, tumors can be induced in a genetically engineered mouse model. In this setting, CCl4 is administered for 12 weeks after tail-vein injection of Cre-expressing adenovirus in Mst1–/–Mst2F/– mice and results in development of HCC tumors (hepatocarcinogenesis) concomitantly to liver cirrhosis.
Published Version: 10.1038/nprot.2015.080
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:23681563
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters