Maturation of mast cell progenitors to mucosal mast cells during allergic pulmonary inflammation in mice
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CitationBankova, LG, DF Dwyer, AY Liu, KF Austen, and MF Gurish. 2014. “Maturation of mast cell progenitors to mucosal mast cells during allergic pulmonary inflammation in mice.” Mucosal immunology 8 (3): 596-606. doi:10.1038/mi.2014.91. http://dx.doi.org/10.1038/mi.2014.91.
AbstractIn contrast to resident constitutive mast cells (CMCs), mucosal MCs (MMCs) appear in lung and trachea of sensitized mice only following inhalation challenge. We monitored the influx and maturation of MCs by their expression of Kit, FcεRI, β7 integrin and side scatter (SSC) by flow cytometry. Influx of MC progenitors (MCps) (FcεRIlo, Kitint, β7hi, SSClo) peaks 1 day after challenges and subsides to baseline by day 7 post-challenge. The mature MMCs appear as a distinct population on day 7 and peak at day 14 with higher SSC and FcεRI expression, but lower β7 and Kit expression. A distinct transitional population is present between 1 and 7 days post-challenge. Maturation occurs more rapidly in the trachea. The resident tracheal CMC had higher SSC, FcεRI, and Kit and lower β7 integrin expression than the MMC. By histology, the MMCs follow similar kinetics to the flow cytometry-identified mature MMCs and are notably persistent for >42 days. Steroid treatment reduced inflammation and MCp influx but had no effect on established MMC. Thus changes in SSC, FcεRI, and Kit together with expression of αE/α4:β7 integrins characterizes the development of induced MMCs from MCps and distinguishes them from resident CMC in the trachea and large airways.
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