Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow

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Author
Yu, Vionnie W.C.
Cook, Colleen
Lotinun, Sutada
Pardo-Saganta, Ana
Wang, Ying-Hua
Lymperi, Stefania
Ferraro, Francesca
Raaijmakers, Marc H.G.P.
Wu, Joy Y.
Zhou, Lan
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1084/jem.20141843Metadata
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Yu, V. W., B. Saez, C. Cook, S. Lotinun, A. Pardo-Saganta, Y. Wang, S. Lymperi, et al. 2015. “Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow.” The Journal of Experimental Medicine 212 (5): 759-774. doi:10.1084/jem.20141843. http://dx.doi.org/10.1084/jem.20141843.Abstract
Production of the cells that ultimately populate the thymus to generate α/β T cells has been controversial, and their molecular drivers remain undefined. Here, we report that specific deletion of bone-producing osteocalcin (Ocn)-expressing cells in vivo markedly reduces T-competent progenitors and thymus-homing receptor expression among bone marrow hematopoietic cells. Decreased intrathymic T cell precursors and decreased generation of mature T cells occurred despite normal thymic function. The Notch ligand DLL4 is abundantly expressed on bone marrow Ocn+ cells, and selective depletion of DLL4 from these cells recapitulated the thymopoietic abnormality. These data indicate that specific mesenchymal cells in bone marrow provide key molecular drivers enforcing thymus-seeding progenitor generation and thereby directly link skeletal biology to the production of T cell–based adaptive immunity.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419348/pdf/Terms of Use
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