Flow-induced protein kinase A–CREB pathway acts via BMP signaling to promote HSC emergence
Kim, Peter Geon
Rowe, R. Grant
Chou, Stephanie S.
Ross, Samantha J.
Sakamoto, Kathleen M.
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CitationKim, P. G., H. Nakano, P. P. Das, M. J. Chen, R. G. Rowe, S. S. Chou, S. J. Ross, et al. 2015. “Flow-induced protein kinase A–CREB pathway acts via BMP signaling to promote HSC emergence.” The Journal of Experimental Medicine 212 (5): 633-648. doi:10.1084/jem.20141514. http://dx.doi.org/10.1084/jem.20141514.
AbstractFluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in the aorta–gonad–mesonephros (AGM) of the developing mouse embryo. We determined that the AGM is enriched for expression of targets of protein kinase A (PKA)–cAMP response element-binding protein (CREB), a pathway activated by fluid shear stress. By analyzing CREB genomic occupancy from chromatin-immunoprecipitation sequencing (ChIP-seq) data, we identified the bone morphogenetic protein (BMP) pathway as a potential regulator of CREB. By chemical modulation of the PKA–CREB and BMP pathways in isolated AGM VE-cadherin+ cells from mid-gestation embryos, we demonstrate that PKA–CREB regulates hematopoietic engraftment and clonogenicity of hematopoietic progenitors, and is dependent on secreted BMP ligands through the type I BMP receptor. Finally, we observed blunting of this signaling axis using Ncx1-null embryos, which lack a heartbeat and intravascular flow. Collectively, we have identified a novel PKA–CREB–BMP signaling pathway downstream of shear stress that regulates HSC emergence in the AGM via the endothelial-to-hematopoietic transition.
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