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dc.contributor.authorBadran, Ahmed H.en_US
dc.contributor.authorLiu, David R.en_US
dc.date.accessioned2015-12-04T18:15:24Z
dc.date.issued2015en_US
dc.identifier.citationBadran, Ahmed H., and David R. Liu. 2015. “Development of potent in vivo mutagenesis plasmids with broad mutational spectra.” Nature Communications 6 (1): 8425. doi:10.1038/ncomms9425. http://dx.doi.org/10.1038/ncomms9425.en
dc.identifier.issn2041-1723en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23845341
dc.description.abstractMethods to enhance random mutagenesis in cells offer advantages over in vitro mutagenesis, but current in vivo methods suffer from a lack of control, genomic instability, low efficiency and narrow mutational spectra. Using a mechanism-driven approach, we created a potent, inducible, broad-spectrum and vector-based mutagenesis system in E. coli that enhances mutation 322,000-fold over basal levels, surpassing the mutational efficiency and spectra of widely used in vivo and in vitro methods. We demonstrate that this system can be used to evolve antibiotic resistance in wild-type E. coli in <24 h, outperforming chemical mutagens, ultraviolet light and the mutator strain XL1-Red under similar conditions. This system also enables the continuous evolution of T7 RNA polymerase variants capable of initiating transcription using the T3 promoter in <10 h. Our findings enable broad-spectrum mutagenesis of chromosomes, episomes and viruses in vivo, and are applicable to both bacterial and bacteriophage-mediated laboratory evolution platforms.en
dc.language.isoen_USen
dc.publisherNature Pub. Groupen
dc.relation.isversionofdoi:10.1038/ncomms9425en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633624/pdf/en
dash.licenseLAAen_US
dc.titleDevelopment of potent in vivo mutagenesis plasmids with broad mutational spectraen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature Communicationsen
dash.depositing.authorBadran, Ahmed H.en_US
dc.date.available2015-12-04T18:15:24Z
dc.identifier.doi10.1038/ncomms9425*
dash.contributor.affiliatedBadran, Ahmed


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