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dc.contributor.authorDeng, Wenjunen_US
dc.contributor.authorBabu, I. Rameshen_US
dc.contributor.authorSu, Danen_US
dc.contributor.authorYin, Shanyeen_US
dc.contributor.authorBegley, Thomas J.en_US
dc.contributor.authorDedon, Peter C.en_US
dc.date.accessioned2016-01-04T19:23:20Z
dc.date.issued2015en_US
dc.identifier.citationDeng, Wenjun, I. Ramesh Babu, Dan Su, Shanye Yin, Thomas J. Begley, and Peter C. Dedon. 2015. “Trm9-Catalyzed tRNA Modifications Regulate Global Protein Expression by Codon-Biased Translation.” PLoS Genetics 11 (12): e1005706. doi:10.1371/journal.pgen.1005706. http://dx.doi.org/10.1371/journal.pgen.1005706.en
dc.identifier.issn1553-7390en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:23993521
dc.description.abstractPost-transcriptional modifications of transfer RNAs (tRNAs) have long been recognized to play crucial roles in regulating the rate and fidelity of translation. However, the extent to which they determine global protein production remains poorly understood. Here we use quantitative proteomics to show a direct link between wobble uridine 5-methoxycarbonylmethyl (mcm5) and 5-methoxy-carbonyl-methyl-2-thio (mcm5s2) modifications catalyzed by tRNA methyltransferase 9 (Trm9) in tRNAArg(UCU) and tRNAGlu(UUC) and selective translation of proteins from genes enriched with their cognate codons. Controlling for bias in protein expression and alternations in mRNA expression, we find that loss of Trm9 selectively impairs expression of proteins from genes enriched with AGA and GAA codons under both normal and stress conditions. Moreover, we show that AGA and GAA codons occur with high frequency in clusters along the transcripts, which may play a role in modulating translation. Consistent with these results, proteins subject to enhanced ribosome pausing in yeast lacking mcm5U and mcm5s2U are more likely to be down-regulated and contain a larger number of AGA/GAA clusters. Together, these results suggest that Trm9-catalyzed tRNA modifications play a significant role in regulating protein expression within the cell.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pgen.1005706en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689569/pdf/en
dash.licenseLAAen_US
dc.titleTrm9-Catalyzed tRNA Modifications Regulate Global Protein Expression by Codon-Biased Translationen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Geneticsen
dash.depositing.authorYin, Shanyeen_US
dc.date.available2016-01-04T19:23:20Z
dc.identifier.doi10.1371/journal.pgen.1005706*
dash.contributor.affiliatedYin, Shanye


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