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dc.contributor.authorKonstantinopoulos, Panagiotis A.en_US
dc.contributor.authorVandoros, Gerasimos P.en_US
dc.contributor.authorKaramouzis, Michalis V.en_US
dc.contributor.authorGkermpesi, Mariaen_US
dc.contributor.authorSotiropoulou-Bonikou, Georgiaen_US
dc.contributor.authorPapavassiliou, Athanasios G.en_US
dc.date.accessioned2016-02-01T15:45:38Z
dc.date.issued2007en_US
dc.identifier.citationKonstantinopoulos, Panagiotis A., Gerasimos P. Vandoros, Michalis V. Karamouzis, Maria Gkermpesi, Georgia Sotiropoulou-Bonikou, and Athanasios G. Papavassiliou. 2007. “EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF.” Cellular Oncology : the Official Journal of the International Society for Cellular Oncology 29 (6): 477-482. doi:10.1155/2007/831416. http://dx.doi.org/10.1155/2007/831416.en
dc.identifier.issn1570-5870en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:24983928
dc.description.abstractBackground:: COX-2 and VEGF are important triggers of colon cancer growth, metastasis and angiogenesis. Cox-2 promoter contains transcriptional regulatory elements for AP-1 and NF-κ:B transcription factors whilst vegf is a known AP-1 downstream target gene. We investigated whether stromal myofibroblasts surrounding colon adenocarcinomas express COX-2 and VEGF and whether activation of AP-1 and NF-κ:B, as well as expression of EGF-R parallel expression of COX-2 and VEGF in these cells. Methods:: Immunohistochemical methodology was performed on archival sections from 40 patients with colon adenocarcinomas. We evaluated c-FOS, p-c-JUN (phosphorylated c-JUN), p-Iκ:B-α (phosphorylated Iκ:B-α), EGF-R, COX-2, NF-κ:B and VEGF expression in stromal myofibroblasts surrounding colon adenocarcinomas. Double immunostaining with a-smooth muscle actin and each antibody was done to verify the expression of these molecules in stromal myofibroblasts. Results:: VEGF, p-Iκ:B-α, NF-κ:B, c-FOS, p-c-JUN, EGF-R and COX-2 were expressed in stromal myofibroblasts surrounding colon adenocarcinomas in the majority of cases. EGF-R, p-Iκ:B-α, NF-κ:B, c-FOS and p-c-JUN correlated positively with COX-2 and VEGF expression. Conclusion:: Stromal myofibroblasts surrounding colon adenocarcinomas are an important source of VEGF and COX-2 production, while AP-1 and NF-κ:B transcription factors are activated and EGF-R is expressed in these cells and associated with COX-2 and VEGF production.en
dc.language.isoen_USen
dc.publisherIOS Pressen
dc.relation.isversionofdoi:10.1155/2007/831416en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618411/pdf/en
dash.licenseLAAen_US
dc.subjectColon adenocarcinomaen
dc.subjectstromal myofibroblastsen
dc.subjectEGF-Ren
dc.subjectAP-1en
dc.subjectNF-en
dc.subjectCOX-2en
dc.subjectVEGFen
dc.titleEGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGFen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalCellular Oncology : the Official Journal of the International Society for Cellular Oncologyen
dash.depositing.authorKonstantinopoulos, Panagiotis A.en_US
dc.date.available2016-02-01T15:45:38Z
dc.identifier.doi10.1155/2007/831416*
dash.contributor.affiliatedKonstantinopoulos, Panagiotis


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