Clonal analyses and gene profiling identify genetic biomarkers of human brown and white preadipocyte thermogenic potential
Dreyfuss, Jonathan M.
Schulz, Tim J.
Huang, Tian Lian
Townsend, Kristy L.
Weiner, Lauren S.
Lynes, Maureen S.
Cypess, Aaron M.
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CitationXue, R., M. D. Lynes, J. M. Dreyfuss, F. Shamsi, T. J. Schulz, H. Zhang, T. L. Huang, et al. 2015. “Clonal analyses and gene profiling identify genetic biomarkers of human brown and white preadipocyte thermogenic potential.” Nature medicine 21 (7): 760-768. doi:10.1038/nm.3881. http://dx.doi.org/10.1038/nm.3881.
AbstractTargeting brown adipose tissue (BAT) content or activity has therapeutic potential for treating obesity and the metabolic syndrome by increasing energy expenditure. Both inter- and intra-individual differences contribute to heterogeneity in human BAT and potentially to differential thermogenic capacity in human populations. Here, we demonstrated the generated clones of brown and white preadipocytes from human neck fat of four individuals and characterized their adipogenic differentiation and thermogenic function. Combining an uncoupling protein 1(UCP1) reporter system and expression profiling, we defined novel sets of gene signatures in human preadipocytes that could predict the thermogenic potential of the cells once they were maturated in culture. Knocking out the positive UCP1 regulators identified by this approach, PREX1 and EDNRB in brown preadipocytes using CRISPR/Cas9 markedly abolished the high level of UCP1 in brown adipocytes differentiated from the preadipocytes. Finally, we were able to prospectively isolate adipose progenitors with great thermogenic potential using cell surface marker CD29. These data provide new insights into the cellular heterogeneity in human fat and offer the identification of possible biomarkers of thermogenically competent preadipocytes.
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