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dc.contributor.authorShon, Min Juen_US
dc.contributor.authorCohen, Adam E.en_US
dc.date.accessioned2016-02-01T15:46:32Z
dc.date.issued2016en_US
dc.identifier.citationShon, Min Ju, and Adam E. Cohen. 2016. “Nano-mechanical measurements of protein-DNA interactions with a silicon nitride pulley.” Nucleic Acids Research 44 (1): e7. doi:10.1093/nar/gkv866. http://dx.doi.org/10.1093/nar/gkv866.en
dc.identifier.issn0305-1048en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:24984010
dc.description.abstractProteins adhere to DNA at locations and with strengths that depend on the protein conformation, the underlying DNA sequence and the ionic content of the solution. A facile technique to probe the positions and strengths of protein-DNA binding would aid in understanding these important interactions. Here, we describe a ‘DNA pulley’ for position-resolved nano-mechanical measurements of protein-DNA interactions. A molecule of λ DNA is tethered by one end to a glass surface, and by the other end to a magnetic bead. The DNA is stretched horizontally by a magnet, and a nanoscale knife made of silicon nitride is manipulated to contact, bend and scan along the DNA. The mechanical profile of the DNA at the contact with the knife is probed via nanometer-precision optical tracking of the magnetic bead. This system enables detection of protein bumps on the DNA and localization of their binding sites. We study theoretically the technical requirements to detect mechanical heterogeneities in the DNA itself.en
dc.language.isoen_USen
dc.publisherOxford University Pressen
dc.relation.isversionofdoi:10.1093/nar/gkv866en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705685/pdf/en
dash.licenseLAAen_US
dc.titleNano-mechanical measurements of protein-DNA interactions with a silicon nitride pulleyen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNucleic Acids Researchen
dash.depositing.authorCohen, Adam E.en_US
dc.date.available2016-02-01T15:46:32Z
dc.identifier.doi10.1093/nar/gkv866*
dash.contributor.affiliatedCohen, Adam


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