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dc.contributor.authorBenam, Kambez H
dc.contributor.authorVillenave, Remi
dc.contributor.authorLucchesi, Carolina
dc.contributor.authorVarone, Antonio
dc.contributor.authorHubeau, Cedric
dc.contributor.authorLee, Hyun-Hee
dc.contributor.authorAlves, Stephen E
dc.contributor.authorSalmon, Michael
dc.contributor.authorFerrante, Thomas Charles
dc.contributor.authorWeaver, James C.
dc.contributor.authorBahinski, Anthony
dc.contributor.authorHamilton, Geraldine A
dc.contributor.authorIngber, Donald Elliot
dc.date.accessioned2016-02-26T16:47:27Z
dash.embargo.terms2016-06-22
dc.date.issued2015
dc.identifier.citationBenam, Kambez H, Remi Villenave, Carolina Lucchesi, Antonio Varone, Cedric Hubeau, Hyun-Hee Lee, Stephen E Alves, et al. 2015. “Small Airway-on-a-Chip Enables Analysis of Human Lung Inflammation and Drug Responses in Vitro.” Nat Meth 13 (2) (December 21): 151–157. doi:10.1038/nmeth.3697.en_US
dc.identifier.issn1548-7091en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:25623045
dc.description.abstractHere we describe development of a human ‘lung small airway-on-a-chip’ containing a differentiated, mucociliary, bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which enables analysis of organ-level lung pathophysiology in vitro. Exposure of the epithelium to IL-13 reconstitutes the goblet cell hyperplasia, cytokine hypersecretion and decreased ciliary function of asthmatics. Small airway chips lined by epithelial cells from chronic obstructive pulmonary disease patients recapitulate features of the disease including selective cytokine hypersecretion, increased neutrophil recruitment, and clinical exacerbations by exposure to viral and bacterial infections. Using this robust in vitro method for modeling human lung inflammatory disorders, it is possible to detect synergistic effects of lung endothelium and epithelium on cytokine secretion, identify new biomarkers of disease exacerbation, and measure therapeutic responses to anti-inflammatory compounds that inhibit cytokine-induced recruitment of circulating neutrophils under flow.en_US
dc.description.sponsorshipEngineering and Applied Sciencesen_US
dc.description.sponsorshipOrganismic and Evolutionary Biologyen_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/nmeth.3697en_US
dash.licenseLAA
dc.titleSmall airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitroen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Methodsen_US
dash.depositing.authorIngber, Donald Elliot
dash.waiver2015-11-24
dc.date.available2016-06-22T07:30:46Z
dc.identifier.doi10.1038/nmeth.3697*
workflow.legacycommentsFrom Waiver Tableen_US
dash.authorsorderedfalse
dash.contributor.affiliatedBahinski, Anthony
dash.contributor.affiliatedLucchesi, Carolina
dash.contributor.affiliatedFerrante, Thomas
dash.contributor.affiliatedIngber, Donald
dash.contributor.affiliatedWeaver, James


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