Claudin-2-dependent paracellular channels are dynamically gated

DSpace/Manakin Repository

Claudin-2-dependent paracellular channels are dynamically gated

Citable link to this page


Title: Claudin-2-dependent paracellular channels are dynamically gated
Author: Weber, Christopher R; Liang, Guo Hua; Wang, Yitang; Das, Sudipto; Shen, Le; Yu, Alan S L; Nelson, Deborah J; Turner, Jerrold R

Note: Order does not necessarily reflect citation order of authors.

Citation: Weber, Christopher R, Guo Hua Liang, Yitang Wang, Sudipto Das, Le Shen, Alan S L Yu, Deborah J Nelson, and Jerrold R Turner. 2015. “Claudin-2-dependent paracellular channels are dynamically gated.” eLife 4 (1): e09906. doi:10.7554/eLife.09906.
Full Text & Related Files:
Abstract: Intercellular tight junctions form selectively permeable barriers that seal the paracellular space. Trans-tight junction flux has been measured across large epithelial surfaces, but conductance across individual channels has never been measured. We report a novel trans-tight junction patch clamp technique that detects flux across individual claudin-2 channels within the tight junction of cultured canine renal tubule or human intestinal epithelial monolayers. In both cells, claudin-2 channels display conductances of ~90 pS. The channels are gated, strictly dependent on claudin-2 expression, and display size- and charge-selectivity typical of claudin-2. Kinetic analyses indicate one open and two distinct closed states. Conductance is symmetrical and reversible, characteristic of a passive, paracellular process, and blocked by reduced temperature or site-directed mutagenesis and chemical derivatization of the claudin-2 pore. We conclude that claudin-2 forms gated paracellular channels and speculate that modulation of tight junction channel gating kinetics may be an unappreciated mechanism of barrier regulation. DOI:
Published Version: doi:10.7554/eLife.09906
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search