The Role of Physical Stabilization in Whole Blood Preservation

DSpace/Manakin Repository

The Role of Physical Stabilization in Whole Blood Preservation

Citable link to this page

 

 
Title: The Role of Physical Stabilization in Whole Blood Preservation
Author: Wong, Keith H. K.; Sandlin, Rebecca D.; Carey, Thomas R.; Miller, Kathleen L.; Shank, Aaron T.; Oklu, Rahmi; Maheswaran, Shyamala; Haber, Daniel A.; Irimia, Daniel; Stott, Shannon L.; Toner, Mehmet

Note: Order does not necessarily reflect citation order of authors.

Citation: Wong, K. H. K., R. D. Sandlin, T. R. Carey, K. L. Miller, A. T. Shank, R. Oklu, S. Maheswaran, et al. 2016. “The Role of Physical Stabilization in Whole Blood Preservation.” Scientific Reports 6 (1): 21023. doi:10.1038/srep21023. http://dx.doi.org/10.1038/srep21023.
Full Text & Related Files:
Abstract: The rapid degradation of blood ex vivo imposes logistical limitations on the utilization of blood-borne cells in medical diagnostics and scientific investigations. A fundamental but overlooked aspect in the storage of this fluid tissue is blood settling, which induces physical stress and compaction, aggregates blood cells, and causes collateral damage due to leukocyte activation. Here we show that the polymer Ficoll 70 kDa stabilized blood samples and prevented blood settling over the course of 72 hours, primarily by inhibiting depletion-mediated red blood cell aggregation. Physical stabilization decreased echinocyte formation, improved leukocyte viability, and inhibited the release of neutrophil elastase—a marker of neutrophil extracellular trap formation. In addition, Ficoll-stabilized blood was compatible with common leukocyte enrichment techniques including red blood cell lysis and immunomagnetic purification. This study showed for the first time that blood settling can be prevented using polymers and has implications in diagnostics.
Published Version: doi:10.1038/srep21023
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753451/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:25658383
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters