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dc.contributor.authorJi, Zheen_US
dc.contributor.authorSong, Ruishengen_US
dc.contributor.authorRegev, Aviven_US
dc.contributor.authorStruhl, Kevinen_US
dc.date.accessioned2016-03-01T19:49:35Z
dc.date.issued2015en_US
dc.identifier.citationJi, Zhe, Ruisheng Song, Aviv Regev, and Kevin Struhl. 2015. “Many lncRNAs, 5’UTRs, and pseudogenes are translated and some are likely to express functional proteins.” eLife 4 (1): e08890. doi:10.7554/eLife.08890. http://dx.doi.org/10.7554/eLife.08890.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:25658455
dc.description.abstractUsing a new bioinformatic method to analyze ribosome profiling data, we show that 40% of lncRNAs and pseudogene RNAs expressed in human cells are translated. In addition, ~35% of mRNA coding genes are translated upstream of the primary protein-coding region (uORFs) and 4% are translated downstream (dORFs). Translated lncRNAs preferentially localize in the cytoplasm, whereas untranslated lncRNAs preferentially localize in the nucleus. The translation efficiency of cytoplasmic lncRNAs is nearly comparable to that of mRNAs, suggesting that cytoplasmic lncRNAs are engaged by the ribosome and translated. While most peptides generated from lncRNAs may be highly unstable byproducts without function, ~9% of the peptides are conserved in ORFs in mouse transcripts, as are 74% of pseudogene peptides, 24% of uORF peptides and 32% of dORF peptides. Analyses of synonymous and nonsynonymous substitution rates of these conserved peptides show that some are under stabilizing selection, suggesting potential functional importance. DOI: http://dx.doi.org/10.7554/eLife.08890.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.08890en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739776/pdf/en
dash.licenseLAAen_US
dc.subjectnon-coding RNAsen
dc.subjecttranslationen
dc.subjectribosome profilingen
dc.subject5'UTRen
dc.subjectpseudogeneen
dc.subjectbiological functionen
dc.subjectHumanen
dc.titleMany lncRNAs, 5’UTRs, and pseudogenes are translated and some are likely to express functional proteinsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorJi, Zheen_US
dc.date.available2016-03-01T19:49:35Z
dc.identifier.doi10.7554/eLife.08890*
dash.contributor.affiliatedSong, Ruisheng
dash.contributor.affiliatedJi, Zhe
dash.contributor.affiliatedStruhl, Kevin


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