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dc.contributor.authorSpencer, Sarah Jen_US
dc.contributor.authorTamminen, Manu Ven_US
dc.contributor.authorPreheim, Sarah Pen_US
dc.contributor.authorGuo, Mira Ten_US
dc.contributor.authorBriggs, Adrian Wen_US
dc.contributor.authorBrito, Ilana Len_US
dc.contributor.authorA Weitz, Daviden_US
dc.contributor.authorPitkänen, Leena Ken_US
dc.contributor.authorVigneault, Francoisen_US
dc.contributor.authorVirta, Marko PJuhanien_US
dc.contributor.authorAlm, Eric Jen_US
dc.date.accessioned2016-03-01T19:50:44Z
dc.date.issued2016en_US
dc.identifier.citationSpencer, S. J., M. V. Tamminen, S. P. Preheim, M. T. Guo, A. W. Briggs, I. L. Brito, D. A Weitz, et al. 2016. “Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers.” The ISME Journal 10 (2): 427-436. doi:10.1038/ismej.2015.124. http://dx.doi.org/10.1038/ismej.2015.124.en
dc.identifier.issn1751-7362en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:25658509
dc.description.abstractMany microbial communities are characterized by high genetic diversity. 16S ribosomal RNA sequencing can determine community members, and metagenomics can determine the functional diversity, but resolving the functional role of individual cells in high throughput remains an unsolved challenge. Here, we describe epicPCR (Emulsion, Paired Isolation and Concatenation PCR), a new technique that links functional genes and phylogenetic markers in uncultured single cells, providing a throughput of hundreds of thousands of cells with costs comparable to one genomic library preparation. We demonstrate the utility of our technique in a natural environment by profiling a sulfate-reducing community in a freshwater lake, revealing both known sulfate reducers and discovering new putative sulfate reducers. Our method is adaptable to any conserved genetic trait and translates genetic associations from diverse microbial samples into a sequencing library that answers targeted ecological questions. Potential applications include identifying functional community members, tracing horizontal gene transfer networks and mapping ecological interactions between microbial cells.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/ismej.2015.124en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737934/pdf/en
dash.licenseLAAen_US
dc.titleMassively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markersen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalThe ISME Journalen
dash.depositing.authorGuo, Mira Ten_US
dc.date.available2016-03-01T19:50:44Z
dc.identifier.doi10.1038/ismej.2015.124*
dash.authorsorderedfalse
dash.contributor.affiliatedGuo, Mira
dash.contributor.affiliatedBriggs, Adrian


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