Pancreatic cancer cell-derived IGFBP-3 contributes to muscle wasting

DSpace/Manakin Repository

Pancreatic cancer cell-derived IGFBP-3 contributes to muscle wasting

Citable link to this page


Title: Pancreatic cancer cell-derived IGFBP-3 contributes to muscle wasting
Author: Huang, Xiu-yan; Huang, Zi-li; Yang, Ju-hong; Xu, Yong-hua; Sun, Jiu-Song; Zheng, Qi; Wei, Chunyao; Song, Wei; Yuan, Zhou

Note: Order does not necessarily reflect citation order of authors.

Citation: Huang, Xiu-yan, Zi-li Huang, Ju-hong Yang, Yong-hua Xu, Jiu-Song Sun, Qi Zheng, Chunyao Wei, Wei Song, and Zhou Yuan. 2016. “Pancreatic cancer cell-derived IGFBP-3 contributes to muscle wasting.” Journal of Experimental & Clinical Cancer Research : CR 35 (1): 46. doi:10.1186/s13046-016-0317-z.
Full Text & Related Files:
Abstract: Background: Progressive loss of skeletal muscle, termed muscle wasting, is a hallmark of cancer cachexia and contributes to weakness, reduced quality of life, as well as poor response to therapy. Previous studies have indicated that systemic host inflammatory response regarding tumor development results in muscle wasting. However, how tumor directly regulates muscle wasting via tumor-derived secreted proteins is still largely unknown. Methods: In this study, we performed bioinformatics analysis in two datasets of pancreatic ductal adenocarcinoma, which causes cancer cachexia and muscle wasting with the highest prevalence, and uncovered that IGFBP3, which encodes IGF-binding protein-3 (IGFBP-3), is dramatically up-regulated in pancreatic tumor samples. We also verified the wasting effect of IGFBP-3 on C2C12 muscle cells with biochemical and genetic assays. Results: IGFBP-3 potently leads to impaired myogenesis and enhanced muscle protein degradation, the major features of muscle wasting, via IGF signaling inhibition. Moreover, conditioned medium from Capan-1 pancreatic cancer cells, which contains abundant IGFBP-3, significantly induces muscle cell wasting. This wasting effect is potently alleviated by IGFBP3 knockdown in Capan-1 cells or IGFBP-3 antibody neutralization. Strikingly, compared to muscle cells, IGF signaling and proliferation rate of Capan-1 cells were rarely affected by IGFBP-3 treatment. Conclusions: Our results demonstrated that pancreatic cancer cells induce muscle wasting via IGFBP-3 production. Electronic supplementary material The online version of this article (doi:10.1186/s13046-016-0317-z) contains supplementary material, which is available to authorized users.
Published Version: doi:10.1186/s13046-016-0317-z
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search