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dc.contributor.authorLin, Xiaolinen_US
dc.contributor.authorJia, Junshuangen_US
dc.contributor.authorQin, Yujuanen_US
dc.contributor.authorLin, Xiaen_US
dc.contributor.authorLi, Weien_US
dc.contributor.authorXiao, Gaofangen_US
dc.contributor.authorLi, Yanqingen_US
dc.contributor.authorXie, Raoyingen_US
dc.contributor.authorHuang, Hailuen_US
dc.contributor.authorZhong, Linen_US
dc.contributor.authorWu, Qinghongen_US
dc.contributor.authorWang, Wanshanen_US
dc.contributor.authorHuang, Wenhuaen_US
dc.contributor.authorYao, Kaitaien_US
dc.contributor.authorXiao, Dongen_US
dc.contributor.authorSun, Yanen_US
dc.date.accessioned2016-04-01T15:47:24Z
dc.date.issued2015en_US
dc.identifier.citationLin, X., J. Jia, Y. Qin, X. Lin, W. Li, G. Xiao, Y. Li, et al. 2015. “Simple and rapid determination of homozygous transgenic mice via in vivo fluorescence imaging.” Oncotarget 6 (36): 39073-39087.en
dc.identifier.issn1949-2553en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:26318514
dc.description.abstractSetting up breeding programs for transgenic mouse strains require to distinguish homozygous from the heterozygous transgenic animals. The combinational use of the fluorescence reporter transgene and small animal in-vivo imaging system might allow us to rapidly and visually determine the transgenic mice homozygous for transgene(s) by the in vivo fluorescence imaging. RLG, RCLG or Rm17LG transgenic mice ubiquitously express red fluorescent protein (RFP). To identify homozygous RLG transgenic mice, whole-body fluorescence imaging for all of newborn F2-generation littermates produced by mating of RFP-positive heterozygous transgenic mice (F1-generation) derived from the same transgenic founder was performed. Subsequently, the immediate data analysis of the in vivo fluorescence imaging was carried out, which greatly facilitated us to rapidly and readily distinguish RLG transgenic individual(s) with strong fluorescence from the rest of F2-generation littermates, followed by further determining this/these RLG individual(s) showing strong fluorescence to be homozygous, as strongly confirmed by mouse mating. Additionally, homozygous RCLG or Rm17LG transgenic mice were also rapidly and precisely distinguished by the above-mentioned optical approach. This approach allowed us within the shortest time period to obtain 10, 8 and 2 transgenic mice homozygous for RLG, RCLG and Rm17LG transgene, respectively, as verified by mouse mating, indicating the practicality and reliability of this optical method. Taken together, our findings fully demonstrate that the in vivo fluorescence imaging offers a visual, rapid and reliable alternative method to the traditional approaches (i.e., mouse mating and real-time quantitative PCR) in identifying homozygous transgenic mice harboring fluorescence reporter transgene under the control of a ubiquitous promoter in the situation mentioned in this study.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766372/pdf/en
dash.licenseLAAen_US
dc.subjecttransgenic miceen
dc.subjectfluorescence reporter geneen
dc.subjecthomozygoteen
dc.titleSimple and rapid determination of homozygous transgenic mice via in vivo fluorescence imagingen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dash.depositing.authorSun, Yanen_US
dc.date.available2016-04-01T15:47:24Z
dash.authorsorderedfalse
dash.contributor.affiliatedSun, Yan


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