Regulation at a distance of biomolecular interactions using a DNA origami nanoactuator

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Regulation at a distance of biomolecular interactions using a DNA origami nanoactuator

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Title: Regulation at a distance of biomolecular interactions using a DNA origami nanoactuator
Author: Ke, Yonggang; Meyer, Travis; Shih, William M.; Bellot, Gaetan

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Citation: Ke, Yonggang, Travis Meyer, William M. Shih, and Gaetan Bellot. 2016. “Regulation at a distance of biomolecular interactions using a DNA origami nanoactuator.” Nature Communications 7 (1): 10935. doi:10.1038/ncomms10935. http://dx.doi.org/10.1038/ncomms10935.
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Abstract: The creation of nanometre-sized structures that exhibit controllable motions and functions is a critical step towards building nanomachines. Recent developments in the field of DNA nanotechnology have begun to address these goals, demonstrating complex static or dynamic nanostructures made of DNA. Here we have designed and constructed a rhombus-shaped DNA origami ‘nanoactuator' that uses mechanical linkages to copy distance changes induced on one half (‘the driver') to be propagated to the other half (‘the mirror'). By combining this nanoactuator with split enhanced green fluorescent protein (eGFP), we have constructed a DNA–protein hybrid nanostructure that demonstrates tunable fluorescent behaviours via long-range allosteric regulation. In addition, the nanoactuator can be used as a sensor that responds to specific stimuli, including changes in buffer composition and the presence of restriction enzymes or specific nucleic acids.
Published Version: doi:10.1038/ncomms10935
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802031/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:26318617
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