Endomucin prevents leukocyte–endothelial cell adhesion and has a critical role under resting and inflammatory conditions
View/ Open
Author
Zahr, Alisar
Alcaide, Pilar
Jones, Alexander
Gregory, Meredith
dela Paz, Nathaniel G.
Patel-Hett, Sunita
Nevers, Tania
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/ncomms10363Metadata
Show full item recordCitation
Zahr, A., P. Alcaide, J. Yang, A. Jones, M. Gregory, N. G. dela Paz, S. Patel-Hett, et al. 2016. “Endomucin prevents leukocyte–endothelial cell adhesion and has a critical role under resting and inflammatory conditions.” Nature Communications 7 (1): 10363. doi:10.1038/ncomms10363. http://dx.doi.org/10.1038/ncomms10363.Abstract
Endomucin is a membrane-bound glycoprotein expressed luminally by endothelial cells that line postcapillary venules, a primary site of leukocyte recruitment during inflammation. Here we show that endomucin abrogation on quiescent endothelial cells enables neutrophils to adhere firmly, via LFA-1-mediated binding to ICAM-1 constitutively expressed by endothelial cells. Moreover, TNF-α stimulation downregulates cell surface expression of endomucin concurrent with increased expression of adhesion molecules. Adenovirus-mediated expression of endomucin under inflammatory conditions prevents neutrophil adhesion in vitro and reduces the infiltration of CD45+ and NIMP-R14+ cells in vivo. These results indicate that endomucin prevents leukocyte contact with adhesion molecules in non-inflamed tissues and that downregulation of endomucin is critical to facilitate adhesion of leukocytes into inflamed tissues.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740757/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:26318644
Collections
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)