Chromatin topology is coupled to Polycomb group protein subnuclear organization
Wani, Ajazul H.
Francis, Nicole J.
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CitationWani, Ajazul H., Alistair N. Boettiger, Patrick Schorderet, Ayla Ergun, Christine Münger, Ruslan I. Sadreyev, Xiaowei Zhuang, Robert E. Kingston, and Nicole J. Francis. 2016. “Chromatin topology is coupled to Polycomb group protein subnuclear organization.” Nature Communications 7 (1): 10291. doi:10.1038/ncomms10291. http://dx.doi.org/10.1038/ncomms10291.
AbstractThe genomes of metazoa are organized at multiple scales. Many proteins that regulate genome architecture, including Polycomb group (PcG) proteins, form subnuclear structures. Deciphering mechanistic links between protein organization and chromatin architecture requires precise description and mechanistic perturbations of both. Using super-resolution microscopy, here we show that PcG proteins are organized into hundreds of nanoscale protein clusters. We manipulated PcG clusters by disrupting the polymerization activity of the sterile alpha motif (SAM) of the PcG protein Polyhomeotic (Ph) or by increasing Ph levels. Ph with mutant SAM disrupts clustering of endogenous PcG complexes and chromatin interactions while elevating Ph level increases cluster number and chromatin interactions. These effects can be captured by molecular simulations based on a previously described chromatin polymer model. Both perturbations also alter gene expression. Organization of PcG proteins into small, abundant clusters on chromatin through Ph SAM polymerization activity may shape genome architecture through chromatin interactions.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:26318701
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