A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation
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A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation
| Title: | A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation |
| Author: |
Chetty, Sundari; Engquist, Elise N.; Mehanna, Elie; Lui, Kathy O.; Tsankov, Alexander M.; Melton, Douglas A.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Chetty, Sundari, Elise N. Engquist, Elie Mehanna, Kathy O. Lui, Alexander M. Tsankov, and Douglas A. Melton. 2015. “A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation.” The Journal of Cell Biology 210 (7): 1257-1268. doi:10.1083/jcb.201502035. http://dx.doi.org/10.1083/jcb.201502035. |
| Full Text & Related Files: |
4586752.pdf (13.49Mb; PDF) 
|
| Abstract: | Driving human pluripotent stem cells (hPSCs) into specific lineages is an inefficient and challenging process. We show that a potent Src inhibitor, PP1, regulates expression of genes involved in the G1 to S phase transition of the cell cycle, activates proteins in the retinoblastoma family, and subsequently increases the differentiation propensities of hPSCs into all three germ layers. We further demonstrate that genetic suppression of Src regulates the activity of the retinoblastoma protein and enhances the differentiation potential of hPSCs across all germ layers. These positive effects extend beyond the initial germ layer specification and enable efficient differentiation at subsequent stages of differentiation. |
| Published Version: | doi:10.1083/jcb.201502035 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586752/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:26318710 |
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