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dc.contributor.authorMcGuigan, Alison P.
dc.contributor.authorBruzewicz, Derek A.
dc.contributor.authorGlavan, Ana
dc.contributor.authorButte, Manish
dc.contributor.authorWhitesides, George
dc.date.accessioned2009-03-01T00:40:20Z
dc.date.issued2008
dc.identifier.citationMcGuigan, Alison P., Derek A. Bruzewicz, Ana Glavan, Manish Butte, and George M. Whitesides. 2008. Cell Encapsulation in Sub-mm Sized Gel Modules Using Replica Molding. PLoS ONE 3(5): e2258.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:2640568
dc.description.abstractFor many types of cells, behavior in two-dimensional (2D) culture differs from that in three-dimensional (3D) culture. Among biologists, 2D culture on treated plastic surfaces is currently the most popular method for cell culture. In 3D, no analogous standard method—one that is similarly convenient, flexible, and reproducible—exists. This paper describes a soft-lithographic method to encapsulate cells in 3D gel objects (modules) in a variety of simple shapes (cylinders, crosses, rectangular prisms) with lateral dimensions between 40 and 1000 ?m, cell densities of 105 – 108 cells/cm3, and total volumes between 1×10?7 and 8×10?4 cm3. By varying (i) the initial density of cells at seeding, and (ii) the dimensions of the modules, the number of cells per module ranged from 1 to 2500 cells. Modules were formed from a range of standard biopolymers, including collagen, Matrigel™, and agarose, without the complex equipment often used in encapsulation. The small dimensions of the modules allowed rapid transport of nutrients by diffusion to cells at any location in the module, and therefore allowed generation of modules with cell densities near to those of dense tissues (108 – 109 cells/cm3). This modular method is based on soft lithography and requires little special equipment; the method is therefore accessible, flexible, and well suited to (i) understanding the behavior of cells in 3D environments at high densities of cells, as in dense tissues, and (ii) developing applications in tissue engineering.en
dc.description.sponsorshipChemistry and Chemical Biologyen
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0002258en
dash.licenseLAA
dc.subjectcell biologyen
dc.subjectcell adhesionen
dc.subjectcytoskeletonen
dc.subjectcell growth and divisionen
dc.subjectcellular death and stress responsesen
dc.subjectbioengineeringen
dc.subjectchemical biology of the cellen
dc.subjectbiotechnology
dc.subjectchemical biology
dc.titleCell Encapsulation in Sub-mm Sized Gel Modules Using Replica Moldingen
dc.relation.journalPLoS ONEen
dash.depositing.authorWhitesides, George
dc.identifier.doi10.1371/journal.pone.0002258*
dash.contributor.affiliatedWhitesides, George
dc.identifier.orcid0000-0001-9451-2442


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