The Potential and Challenges of Nanopore Sequencing

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The Potential and Challenges of Nanopore Sequencing

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Title: The Potential and Challenges of Nanopore Sequencing
Author: Branton, Daniel; Pershin, Yuriy V; Deamer, David W; Ling, Xinsheng Sean; Wiggin, Matthew; Lindsay, Stuart; Krstic, Predrag S; Huang, Xiaohua; Jovanovich, Stevan B; Butler, Thomas; Soni, Gautam V; Schloss, Jeffery A; Riehn, Robert; Hibbs, Andrew; Tabard-Cossa, Vincent; Wanunu, Meni; Bayley, Hagan; Marziali, Andre; Ramsey, J Michael; Mastrangelo, Carlos H; Meller, Amit; Benner, Steven A; Oliver, John S; Garaj, Slaven; Di Ventra, Massimiliano

Note: Order does not necessarily reflect citation order of authors.

Citation: Branton, Daniel, David W. Deamer, Andre Marziali, Hagan Bayley, Steven A. Benner, Thomas Butler, Massimiliano Di Ventra, et al. 2008. The potential and challenges of nanopore sequencing. Nature Biotechnology 26(10): 1146-1153.
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Abstract: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced
in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing
a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of ‘third generation’ instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.
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