The Potential and Challenges of Nanopore Sequencing
Pershin, Yuriy V
Deamer, David W
Ling, Xinsheng Sean
Krstic, Predrag S
Jovanovich, Stevan B
Soni, Gautam V
Schloss, Jeffery A
Ramsey, J Michael
Mastrangelo, Carlos H
Benner, Steven A
Oliver, John S
Di Ventra, MassimilianoNote: Order does not necessarily reflect citation order of authors.
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CitationBranton, Daniel, David W. Deamer, Andre Marziali, Hagan Bayley, Steven A. Benner, Thomas Butler, Massimiliano Di Ventra, et al. 2008. The potential and challenges of nanopore sequencing. Nature Biotechnology 26(10): 1146-1153.
AbstractA nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced
in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing
a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of ‘third generation’ instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.
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