Show simple item record

dc.contributor.authorBranton, Daniel
dc.contributor.authorPershin, Yuriy V
dc.contributor.authorDeamer, David W
dc.contributor.authorLing, Xinsheng Sean
dc.contributor.authorWiggin, Matthew
dc.contributor.authorLindsay, Stuart
dc.contributor.authorKrstic, Predrag S
dc.contributor.authorHuang, Xiaohua
dc.contributor.authorJovanovich, Stevan B
dc.contributor.authorButler, Thomas
dc.contributor.authorSoni, Gautam V
dc.contributor.authorSchloss, Jeffery A
dc.contributor.authorRiehn, Robert
dc.contributor.authorHibbs, Andrew
dc.contributor.authorTabard-Cossa, Vincent
dc.contributor.authorWanunu, Meni
dc.contributor.authorBayley, Hagan
dc.contributor.authorMarziali, Andre
dc.contributor.authorRamsey, J Michael
dc.contributor.authorMastrangelo, Carlos H
dc.contributor.authorMeller, Amit
dc.contributor.authorBenner, Steven A
dc.contributor.authorOliver, John S
dc.contributor.authorGaraj, Slaven
dc.contributor.authorDi Ventra, Massimiliano
dc.date.accessioned2009-03-14T16:30:06Z
dc.date.issued2008
dc.identifier.citationBranton, Daniel, David W. Deamer, Andre Marziali, Hagan Bayley, Steven A. Benner, Thomas Butler, Massimiliano Di Ventra, et al. 2008. The potential and challenges of nanopore sequencing. Nature Biotechnology 26(10): 1146-1153.en
dc.identifier.issn1087-0156en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:2664284
dc.description.abstractA nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of ‘third generation’ instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.en
dc.description.sponsorshipMolecular and Cellular Biologyen
dc.description.sponsorshipPhysicsen
dc.language.isoen_USen
dc.publisherMacmillian Publishers Ltd.en
dc.relation.isversionofhttp://dx.doi.org/10.1038/nbt.1495en
dash.licenseLAA
dc.titleThe Potential and Challenges of Nanopore Sequencingen
dc.typeJournal Article
dc.description.versionAccepted Manuscript
dc.relation.journalNature Biotechnologyen
dc.identifier.doi10.1038/nbt.1495*
dash.authorsorderedfalse
dash.contributor.affiliatedBranton, Daniel


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record