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dc.contributor.authorBhatt, Ami Bharat
dc.contributor.authorFreeman, Sam Spenser Sharpe
dc.contributor.authorHerrera, Alex Francisco
dc.contributor.authorPedamallu, Chandra Sekhar
dc.contributor.authorGevers, Dirk
dc.contributor.authorDuke, Fujiko
dc.contributor.authorJung, Joonil
dc.contributor.authorMichaud, Monia
dc.contributor.authorWalker, Bruce David
dc.contributor.authorYoung, Sally U.
dc.contributor.authorEarl, Ashlee M.
dc.contributor.authorKostic, Aleksander D.
dc.contributor.authorOjesina, Akinyemi Ifedapo
dc.contributor.authorHasserjian, Robert Paul
dc.contributor.authorBallen, Karen Kuhn
dc.contributor.authorChen, Yi-Bin Albert
dc.contributor.authorHobbs, Gabriela
dc.contributor.authorAntin, Joseph Harry
dc.contributor.authorSoiffer, Robert Jon
dc.contributor.authorBaden, Lindsey Robert
dc.contributor.authorGarrett, Wendy S.
dc.contributor.authorHornick, Jason L.
dc.contributor.authorMarty, Francisco Miguel
dc.contributor.authorMeyerson, Matthew Langer
dc.date.accessioned2016-04-26T17:48:51Z
dc.date.issued2013
dc.identifier.citationBhatt, Ami S., Samuel S. Freeman, Alex F. Herrera, Chandra Sekhar Pedamallu, Dirk Gevers, Fujiko Duke, Joonil Jung, et al. 2013. “ Sequence-Based Discovery of Bradyrhizobium Enterica in Cord Colitis Syndrome .” N Engl J Med 369 (6) (August 8): 517–528. doi:10.1056/nejmoa1211115.en_US
dc.identifier.issn0028-4793en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:26835590
dc.description.abstractBACKGROUND—Immunosuppression is associated with a variety of idiopathic clinical syndromes that may have infectious causes. It has been hypothesized that the cord colitis syndrome, a complication of umbilical-cord hematopoietic stem-cell transplantation, is infectious in origin. METHODS—We performed shotgun DNA sequencing on four archived, paraffin-embedded endoscopic colon-biopsy specimens obtained from two patients with cord colitis. Computational subtraction of human and known microbial sequences and assembly of residual sequences into a bacterial draft genome were performed. We used polymerase-chain-reaction (PCR) assays and fluorescence in situ hybridization to determine whether the corresponding bacterium was present in additional patients and controls. RESULTS—DNA sequencing of the biopsy specimens revealed more than 2.5 million sequencing reads that did not match known organisms. These sequences were computationally assembled into a 7.65-Mb draft genome showing a high degree of homology with genomes of bacteria in the bradyrhizobium genus. The corresponding newly discovered bacterium was provisionally named Bradyrhizobium enterica. PCR identified B. enterica nucleotide sequences in biopsy specimens from all three additional patients with cord colitis whose samples were tested, whereas B. enterica sequences were absent in samples obtained from healthy controls and patients with colon cancer or graft-versus-host disease. CONCLUSIONS—We assembled a novel bacterial draft genome from the direct sequencing of tissue specimens from patients with cord colitis. Association of these sequences with cord colitis suggests that B. enterica may be an opportunistic human pathogen.en_US
dc.language.isoen_USen_US
dc.publisherNew England Journal of Medicine (NEJM/MMS)en_US
dc.relation.isversionofdoi:10.1056/NEJMoa1211115en_US
dash.licenseLAA
dc.titleSequence-Based Discovery of Bradyrhizobium enterica in Cord Colitis Syndromeen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNew England Journal of Medicineen_US
dash.depositing.authorGarrett, Wendy S.
dc.date.available2016-04-26T17:48:51Z
dc.identifier.doi10.1056/NEJMoa1211115*
dash.authorsorderedfalse
dash.contributor.affiliatedBhatt, Ami
dash.contributor.affiliatedHerrera, Alex Francisco
dash.contributor.affiliatedBallen, Karen Kuhn
dash.contributor.affiliatedSoiffer, Robert
dash.contributor.affiliatedAntin, Joseph
dash.contributor.affiliatedHobbs, Gabriela
dash.contributor.affiliatedMichaud, Monia
dash.contributor.affiliatedYoung, Sarah
dash.contributor.affiliatedChen, Yi-Bin
dash.contributor.affiliatedBaden, Lindsey
dash.contributor.affiliatedGarrett, Wendy
dash.contributor.affiliatedHasserjian, Robert
dash.contributor.affiliatedPedamallu, Chandra Sekhar
dash.contributor.affiliatedFreeman, Sam
dash.contributor.affiliatedOjesina, Akinyemi Ifedapo
dash.contributor.affiliatedHornick, Jason
dash.contributor.affiliatedMarty, Francisco
dash.contributor.affiliatedWalker, Bruce
dash.contributor.affiliatedMeyerson, Matthew
dc.identifier.orcid0000-0001-6122-9245
dc.identifier.orcid0000-0002-9133-8108


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