dc.contributor.author | Zhang, Limin | en_US |
dc.contributor.author | Cen, Luan | en_US |
dc.contributor.author | Qu, Shaogang | en_US |
dc.contributor.author | Wei, Lei | en_US |
dc.contributor.author | Mo, Mingshu | en_US |
dc.contributor.author | Feng, Junmin | en_US |
dc.contributor.author | Sun, Congcong | en_US |
dc.contributor.author | Xiao, Yousheng | en_US |
dc.contributor.author | Luo, Qin | en_US |
dc.contributor.author | Li, Shaomin | en_US |
dc.contributor.author | Yang, Xinling | en_US |
dc.contributor.author | Xu, Pingyi | en_US |
dc.date.accessioned | 2016-05-02T17:00:15Z | |
dc.date.issued | 2016 | en_US |
dc.identifier.citation | Zhang, L., L. Cen, S. Qu, L. Wei, M. Mo, J. Feng, C. Sun, et al. 2016. “Enhancing Beta-Catenin Activity via GSK3beta Inhibition Protects PC12 Cells against Rotenone Toxicity through Nurr1 Induction.” PLoS ONE 11 (4): e0152931. doi:10.1371/journal.pone.0152931. http://dx.doi.org/10.1371/journal.pone.0152931. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:26860118 | |
dc.description.abstract | Parkinson’s disease (PD) is characterized by progressive degeneration of dopaminergic (DA) neurons in the substantial nigra pars compacta. Increasing evidence showed that Wnt/β-catenin pathway and the orphan nuclear receptor Nurr1 play crucial roles in the survival and functional maintenance of DA neurons in the midbrain and GSK-3β antagonists LiCl and SB216763 were used to activate Wnt/β-catenin pathway experimentally. However, the detail mechanism underlying the neuroprotection against apoptosis on DA neuron is still unclear and the interaction between Wnt/β-catenin and Nurr1 remains undisclosed. In this study, using cell biological assay we investigated the function of Wnt/β-catenin and its crosstalk with Nurr1 on the course of PC12 cell degeneration in vitro. Our data showed that PC12 cell viability was inhibited by rotenone, but attenuated by GSK-3β antagonists LiCl or SB216763. The activity of Wnt/β-catenin pathway was deregulated on exposure of rotenone in a concentration-dependent manner. After the interference of β-catenin with siRNA, LiCl or SB216763 failed to protect PC12 cells from apoptosis by the rotenone toxicity. Our data confirmed that Wnt/β-catenin signaling activated by LiCl or SB216763 enhanced Nurr1 expression to 2.75 ± 0.55 and 4.06 ± 0.41 folds respectively compared with control detected by real-time PCR and the interaction of β-catenin with Nurr1 was identified by co-immunoprecipitate analysis. In conclusion, the data suggested that Wnt/β-catenin and Nurr1 are crucial factors in the survival of DA neurons, and the activation of Wnt/β-catenin pathway exerts protective effects on DA neurons partly by mean of a co-active pattern with Nurr1. This finding may shed a light on the potential treatment of Parkinson disease. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0152931 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821554/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and life sciences | en |
dc.subject | Genetics | en |
dc.subject | Gene expression | en |
dc.subject | Gene regulation | en |
dc.subject | Small interfering RNAs | en |
dc.subject | Biochemistry | en |
dc.subject | Nucleic acids | en |
dc.subject | RNA | en |
dc.subject | Non-coding RNA | en |
dc.subject | Biology and Life Sciences | en |
dc.subject | Cell Biology | en |
dc.subject | Cellular Types | en |
dc.subject | Animal Cells | en |
dc.subject | Neurons | en |
dc.subject | Neuroscience | en |
dc.subject | Cellular Neuroscience | en |
dc.subject | Medicine and Health Sciences | en |
dc.subject | Neurology | en |
dc.subject | Neurodegenerative Diseases | en |
dc.subject | Movement Disorders | en |
dc.subject | Parkinson Disease | en |
dc.subject | Developmental Biology | en |
dc.subject | Cell Differentiation | en |
dc.subject | Neuronal Differentiation | en |
dc.subject | Precipitation Techniques | en |
dc.subject | Immunoprecipitation | en |
dc.subject | Co-Immunoprecipitation | en |
dc.subject | Neurochemistry | en |
dc.subject | Neurochemicals | en |
dc.subject | Dopaminergics | en |
dc.subject | Anatomy | en |
dc.subject | Brain | en |
dc.subject | Brainstem | en |
dc.subject | Midbrain | en |
dc.title | Enhancing Beta-Catenin Activity via GSK3beta Inhibition Protects PC12 Cells against Rotenone Toxicity through Nurr1 Induction | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Li, Shaomin | en_US |
dc.date.available | 2016-05-02T17:00:15Z | |
dc.identifier.doi | 10.1371/journal.pone.0152931 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Li, Shaomin | |