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dc.contributor.authorIslam, Kamrulen_US
dc.contributor.authorSayeed, Md. Abuen_US
dc.contributor.authorHossen, Emranen_US
dc.contributor.authorKhanam, Farhanaen_US
dc.contributor.authorCharles, Richelle C.en_US
dc.contributor.authorAndrews, Jasonen_US
dc.contributor.authorRyan, Edward T.en_US
dc.contributor.authorQadri, Firdausien_US
dc.date.accessioned2016-05-02T17:02:17Z
dc.date.issued2016en_US
dc.identifier.citationIslam, Kamrul, Md. Abu Sayeed, Emran Hossen, Farhana Khanam, Richelle C. Charles, Jason Andrews, Edward T. Ryan, and Firdausi Qadri. 2016. “Comparison of the Performance of the TPTest, Tubex, Typhidot and Widal Immunodiagnostic Assays and Blood Cultures in Detecting Patients with Typhoid Fever in Bangladesh, Including Using a Bayesian Latent Class Modeling Approach.” PLoS Neglected Tropical Diseases 10 (4): e0004558. doi:10.1371/journal.pntd.0004558. http://dx.doi.org/10.1371/journal.pntd.0004558.en
dc.identifier.issn1935-2727en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:26860315
dc.description.abstractBackground: There is an urgent need for an improved diagnostic assay for typhoid fever. In this current study, we compared the recently developed TPTest (Typhoid and Paratyphoid Test) with the Widal test, blood culture, and two commonly used commercially available kits, Tubex and Typhidot. Methodology For analysis, we categorized 92 Bangladeshi patients with suspected enteric fever into four groups: S. Typhi bacteremic patients (n = 28); patients with a fourfold change in Widal test from day 0 to convalescent period (n = 7); patients with Widal titer ≥1:320 (n = 13) at either acute or convalescent stage of disease; and patients suspected with enteric fever, but with a negative blood culture and Widal titer (n = 44). We also tested healthy endemic zone controls (n = 20) and Bangladeshi patients with other febrile illnesses (n = 15). Sample size was based on convenience to facilitate preliminary analysis. Principle findings Of 28 S. Typhi bacteremic patients, 28 (100%), 21 (75%) and 18 (64%) patients were positive by TPTest, Tubex and Typhidot, respectively. In healthy endemic zone controls, the TPTest method was negative in all, whereas Tubex and Typhidot were positive in 3 (15%) and 5 (25%), respectively. We then estimated sensitivity and specificity of all diagnostic tests using Bayesian latent class modeling. The sensitivity of TPTest, Tubex and Typhidot were estimated at 96.0% (95% CI: 87.1%-99.8%), 60.2% (95% CI: 49.3%-71.2%), and 59.6% (95% CI: 50.1%-69.3%), respectively. Specificity was estimated at 96.6% (90.7%-99.2%) for TPTest, 89.9% (79.6%-96.8%) for Tubex, and 80.0% (67.7%-89.7%) for Typhidot. Conclusion: These results suggest that the TPTest is highly sensitive and specific in diagnosing individuals with typhoid fever in a typhoid endemic setting, outperforming currently available and commonly used alternatives.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pntd.0004558en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825986/pdf/en
dash.licenseLAAen_US
dc.subjectMedicine and Health Sciencesen
dc.subjectDiagnostic Medicineen
dc.subjectSigns and Symptomsen
dc.subjectFeversen
dc.subjectPathology and Laboratory Medicineen
dc.subjectBiology and Life Sciencesen
dc.subjectAnatomyen
dc.subjectBody Fluidsen
dc.subjectBlooden
dc.subjectPhysiologyen
dc.subjectHematologyen
dc.subjectInfectious Diseasesen
dc.subjectBacterial Diseasesen
dc.subjectTyphoiden
dc.subjectSalmonellaen
dc.subjectSalmonella Typhien
dc.subjectMicrobiologyen
dc.subjectMedical Microbiologyen
dc.subjectMicrobial Pathogensen
dc.subjectBacterial Pathogensen
dc.subjectPathogensen
dc.subjectOrganismsen
dc.subjectBacteriaen
dc.subjectEnterobacteriaceaeen
dc.subjectWidal Testen
dc.subjectSerologyen
dc.subjectImmunologic Techniquesen
dc.subjectImmunoassaysen
dc.subjectEnzyme-Linked Immunoassaysen
dc.subjectBiological Culturesen
dc.subjectMicrobial Culturesen
dc.titleComparison of the Performance of the TPTest, Tubex, Typhidot and Widal Immunodiagnostic Assays and Blood Cultures in Detecting Patients with Typhoid Fever in Bangladesh, Including Using a Bayesian Latent Class Modeling Approachen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Neglected Tropical Diseasesen
dash.depositing.authorCharles, Richelle C.en_US
dc.date.available2016-05-02T17:02:17Z
dc.identifier.doi10.1371/journal.pntd.0004558*
dash.contributor.affiliatedCharles, Richelle
dash.contributor.affiliatedRyan, Edward


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