Pooled‐matrix protein interaction screens using Barcode Fusion Genetics
Mellor, Joseph C
Ozturk, Sedide B
Cote, Atina G
Knapp, Jennifer J
Roth, Jonathan F
Roth, Frederick PNote: Order does not necessarily reflect citation order of authors.
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CitationYachie, N., E. Petsalaki, J. C. Mellor, J. Weile, Y. Jacob, M. Verby, S. B. Ozturk, et al. 2016. “Pooled‐matrix protein interaction screens using Barcode Fusion Genetics.” Molecular Systems Biology 12 (4): 863. doi:10.15252/msb.20156660. http://dx.doi.org/10.15252/msb.20156660.
AbstractAbstract High‐throughput binary protein interaction mapping is continuing to extend our understanding of cellular function and disease mechanisms. However, we remain one or two orders of magnitude away from a complete interaction map for humans and other major model organisms. Completion will require screening at substantially larger scales with many complementary assays, requiring further efficiency gains in proteome‐scale interaction mapping. Here, we report Barcode Fusion Genetics‐Yeast Two‐Hybrid (BFG‐Y2H), by which a full matrix of protein pairs can be screened in a single multiplexed strain pool. BFG‐Y2H uses Cre recombination to fuse DNA barcodes from distinct plasmids, generating chimeric protein‐pair barcodes that can be quantified via next‐generation sequencing. We applied BFG‐Y2H to four different matrices ranging in scale from ~25 K to 2.5 M protein pairs. The results show that BFG‐Y2H increases the efficiency of protein matrix screening, with quality that is on par with state‐of‐the‐art Y2H methods.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27320197
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