Noninvasive Tracking of Gene Transcript and Neuroprotection after Gene Therapy
View/ Open
Author
Ren, Jiaqian
Chen, Y. Iris
Liu, Christina H.
Chen, Po-Chih
Prentice, Howard
Wu, Jang-Yen
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/gt.2015.81Metadata
Show full item recordCitation
Ren, Jiaqian, Y. Iris Chen, Christina H. Liu, Po-Chih Chen, Howard Prentice, Jang-Yen Wu, and Philip K. Liu. 2015. “Noninvasive Tracking of Gene Transcript and Neuroprotection after Gene Therapy.” Gene therapy 23 (1): 1-9. doi:10.1038/gt.2015.81. http://dx.doi.org/10.1038/gt.2015.81.Abstract
Gene therapy holds exceptional potential for translational medicine by improving the products of defective genes in diseases and/or providing necessary biologics from endogenous sources during recovery processes. However, validating methods for the delivery, distribution and expression of the exogenous genes from such therapy can generally not be applicable to monitor effects over the long term because they are invasive. We report here that human granulocyte colony-stimulating factor (hG-CSF) cDNA encoded in scAAV-type 2 adeno-associated virus, as delivered through eye drops at multiple time points after cerebral ischemia using bilateral carotid occlusion for 60 min (BCAO-60) led to significant reduction in mortality rates, cerebral atrophy, and neurological deficits in C57black6 mice. Most importantly, we validated hG-CSF cDNA expression using translatable magnetic resonance imaging (MRI) in living brains. This noninvasive approach for monitoring exogenous gene expression in the brains has potential for great impact in the area of experimental gene therapy in animal models of heart attack, stroke, Alzheimer’s dementia, Parkinson’s disorder and amyotrophic lateral sclerosis, and the translation of such techniques to emergency medicine.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706495/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:27320236
Collections
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)