Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition

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Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition

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Title: Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition
Author: Rong, Xiao-Xiang; Wei, Fang; Lin, Xiao-Lin; Qin, Yu-Juan; Chen, Lin; Wang, Hui-Yan; Shen, Hong-Fen; Jia, Li-Ting; Xie, Rao-Ying; Lin, Tao-Yan; Hao, Wei-Chao; Yang, Jie; Yang, Sheng; Cheng, Yu-Shuang; Huang, Wen-Hua; Li, Ai-min; Sun, Yan; Luo, Rong-Cheng; Xiao, Dong

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Citation: Rong, X., F. Wei, X. Lin, Y. Qin, L. Chen, H. Wang, H. Shen, et al. 2016. “Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition.” Oncoimmunology 5 (3): e1086060. doi:10.1080/2162402X.2015.1086060. http://dx.doi.org/10.1080/2162402X.2015.1086060.
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Abstract: ABSTRACT There is an urgent need for more potent and safer approaches to eradicate cancer stem cells (CSCs) for curing cancer. In this study, we investigate cancer-killing activity (CKA) of cytokine-induced killer (CIK) cells against CSCs of hepatocellular carcinoma (HCC). To visualize CSCs in vitro by fluorescence imaging, and image and quantify CSCs in tumor xenograft-bearing mice by bioluminescence imaging, HCC cells were engineered with CSC detector vector encoding GFP and luciferase controlled by Nanog promoter. We found that CIK cells have a strong CKA in vitro against putative CSCs of HCC, as shown by tumorsphere formation and time-lapse imaging. Additionally, time-lapse recording firstly revealed that putative CSCs were attacked simultaneously by many CIK cells and finally eradicated by CIK cells, indicating the necessity of achieving sufficient effector-to-target ratios. We firstly illustrated that anti-NKG2D antibody blocking partially but significantly inhibited CKA of CIK cells against putative CSCs. More importantly, intravenous infusion of CIK cells remarkably delayed tumor growth in mice with a significant decrease in putative CSC number monitored by bioluminescence imaging. Taken together, these findings demonstrate CKA of CIK cells against putative CSCs of HCC, at least in part, by NKG2D-ligands recognition.
Published Version: doi:10.1080/2162402X.2015.1086060
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839362/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:27320249
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