Use of 18F-2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer
View/ Open
Author
Rashidian, Mohammad
Keliher, Edmund J.
Juras, Patrick
K.
Cavallari, Marco
Wojtkiewicz, Gregory R.
Jacobsen, Johanne T.
Edens, Jerre G.
Tas, Jeroen M. J.
Victora, Gabriel
Ploegh, Hidde
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1021/acscentsci.5b00121Metadata
Show full item recordCitation
Rashidian, M., E. J. Keliher, M. Dougan, P. Juras, M. Cavallari, G. R. Wojtkiewicz, J. T. Jacobsen, et al. 2015. “Use of 18F-2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer.” ACS Central Science 1 (3): 142-147. doi:10.1021/acscentsci.5b00121. http://dx.doi.org/10.1021/acscentsci.5b00121.Abstract
We generated 18F-labeled antibody fragments for positron emission tomography (PET) imaging using a sortase-mediated reaction to install a trans-cyclooctene-functionalized short peptide onto proteins of interest, followed by reaction with a tetrazine-labeled-18F-2-deoxyfluoroglucose (FDG). The method is rapid, robust, and site-specific (radiochemical yields > 25%, not decay corrected). The availability of 18F-2-deoxyfluoroglucose avoids the need for more complicated chemistries used to generate carbon–fluorine bonds. We demonstrate the utility of the method by detecting heterotopic pancreatic tumors in mice by PET, using anti-Class II MHC single domain antibodies. We correlate macroscopic PET images with microscopic two-photon visualization of the tumor. Our approach provides easy access to 18F-labeled antibodies and their fragments at a level of molecular specificity that complements conventional 18F-FDG imaging.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778250/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:27320328
Collections
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)