An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells
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Author
Chetty, Sundari
Gifford, Casey A.
Daheron, Laurence
Tsankova, Nadejda M.
Published Version
https://doi.org/10.1038/nbt.3387Metadata
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Tsankov, Alexander M., Veronika Akopian, Ramona Pop, Sundari Chetty, Casey A. Gifford, Laurence Daheron, Douglas A. Melton, Nadejda M. Tsankova, and Alexander Meissner. 2015. “An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells.” Nature biotechnology 33 (11): 1182-1192. doi:10.1038/nbt.3387. http://dx.doi.org/10.1038/nbt.3387.Abstract
Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We have previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. We provide an in-depth characterization of the revised signature panel through embryoid body and directed differentiation experiments as well as a detailed comparison to the teratoma assay. We also show that the improved ScoreCard enables applications such as screening of small molecules, genetic perturbations and assessment of culture conditions. Beyond stem cell applications, this approach can in principle be extended to other cell types and lineages.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636964/pdf/Terms of Use
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