Effect of type of dietary fat and ethanol on antioxidant enzyme mRNA induction in rat liver.

DSpace/Manakin Repository

Effect of type of dietary fat and ethanol on antioxidant enzyme mRNA induction in rat liver.

Citable link to this page

 

 
Title: Effect of type of dietary fat and ethanol on antioxidant enzyme mRNA induction in rat liver.
Author: Nanji, A. A.; Griniuviene, B.; Sadrzadeh, S. M.; Levitsky, Sidney; McCully, James Donald

Note: Order does not necessarily reflect citation order of authors.

Citation: Nanji, A. A., B. Griniuviene, S. M. Sadrzadeh, S. Levitsky, and J. D. McCully. "Effect of type of dietary fat and ethanol on antioxidant enzyme mRNA induction in rat liver." Journal of lipid research 36, no. 4 (1995): 736-744.
Full Text & Related Files:
Abstract: We carried out a study to relate the effect of the type of dietary fat and ethanol on antioxidant enzyme mRNA levels in liver in the intragastric feeding rat model. Different types of dietary fat were administered [saturated fat (SE), corn oil (CE) and fish oil (FE)] with ethanol to induce varying severities of liver injury. Ethanol-fed rats were pair-fed with dextrose-fed controls that received isocaloric amounts of dextrose. All animals were killed at 1 month and the following studies were carried out: evaluation of severity of pathologic liver injury, mRNA quantitation for catalase, glutathione peroxidase (GPx), and manganese superoxide dismutase (MnSOD), microsomal conjugated dienes, and hydrogen peroxide. SE animals had no liver injury, FE animals had severe liver injury, and CE animals had moderate liver injury. Ethanol induced GPx mRNA in all dietary groups, with the highest levels seen in the FE group. The pattern of catalase mRNA induction was similar to that of GPx mRNA. In contrast, MnSOD mRNA was decreased compared to controls in animals that developed pathologic liver injury, i.e., CE and FE groups. A positive correlation was seen between conjugated diene levels and GPx mRNA (r = 0.88, P < 0.01) and catalase mRNA. The similar slopes for the relationship between conjugated dienes and catalase in the fish oil and non-fish oil groups indicate that the same degree of lipid peroxidation increases catalase mRNA to a greater degree in fish oil-fed rats. A positive correlation was also seen between catalase mRNA and H2O2 (r = 0.95, P < 0.001).
Published Version: http://www.jlr.org/content/36/4/736.short
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:27357338
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters